基于形态标记与SRAP标记的莱豆种质资源遗传多样性分析  

Morphological and SRAP Markers-based Genetic Diversity Determination on Phaseolus lunatus L.Germplasms

作　　者：郭媛贞[1] 黄强[1] 叶新如 陈芝[1] 黄枝 陈海玲[1] 颜墩炜 薛珠政[2] GUO Yuanzhen;HUANG Qiang;YE Xinru;CHEN Zhi;HUANG Zhi;CHEN Hailing;YAN Dunwei;XUE Zhuzheng(Putian Institute of Agricultural Science,Putian,Fujian 351100,China;Crops Research Institute,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350013,China)

机构地区：[1]莆田市农业科学研究所,福建莆田351100 [2]福建省农业科学院作物研究所,福建福州350013

出　　处：《福建农业学报》2023年第2期166-173,共8页Fujian Journal of Agricultural Sciences

基　　金：福建省科技计划引导性项目(2020N0044)。

摘　　要：[目的]探明莱豆种质资源遗传多样性和亲缘关系,为莱豆种质资源优良基因深度发掘和新品种选育提供科学依据。[方法]利用形态标记和SRAP分子标记两种方法对22份莱豆资源的26个数量性状和18个质量性状进行测定、分析。[结果]筛选出的28对SRAP引物扩增多态性条带158条,平均多态性比率为77.75%。两种标记方法聚类结果显示,根据莱豆荚果大小可以将22份莱豆资源分为三大类群体。其中,“上横山10-2-6”和“下横山10-3-3”亲缘关系较近,推测可能存在频繁的基因交流。[结论]22份莱豆资源遗传多样性丰富,形态标记和SRAP分子标记两种聚类方法基本支持根据荚果大小划分莱豆资源,为莱豆种质资源的创新利用奠定基础。【Objective】Genetic diversity and relationship of Phaseolus lunatus L.germplasms were determined using the morphological and SRAP markers.【Method】Twenty-six quantitative and 18 quality traits of 22 lima bean germplasms were analyzed based on the morphological as well as the SRAP molecular markers.【Result】Twenty-eight pairs of SRAP primers were selected to obtain 158 amplified polymorphic bands with an average polymorphism ratio of 77.75%.The two methods applying different markers clustered the 22 varieties into 3 categories.Genetically,Shanghengshan10-2-6 and Xiahengshan 10-3-3 were relatively close.They might have gone through numerous gene exchanges in the past.【Conclusion】The 22 lima bean germplasms were rich in genetic diversity.The morphological and SRAP molecular markers-based clustering essentially agreed with the classification by the pod size.

关 键 词：莱豆 形态标记 SRAP分子标记 遗传多样性 亲缘关系 

分 类 号：S643[农业科学—蔬菜学]