针灸调控STAT3和CyclinD1改善DDP小鼠肝损伤的作用机制  

Action Mechanism of Acupuncture and Moxibustion in Improving LiverInjury Induced by DDP by Regulating STAT3 and CyclinD1 in Mice

作　　者：王文哲 于冬冬[1,2] 张晨[1] 魏星宇 杜雪源 庄语 韩乐 王建明 WANG Wenzhe;YU Dongdong;ZHANG Chen;WEI Xingyu;DU Xueyuan;ZHUANG Yu;HAN Le;WANG Jianming(Henan University of Chinese Medicine,Zhengzhou 450046,China;The Third Affiliated Hospital of Henan University of Chinese Medicine,Zhengzhou 450003,China)

机构地区：[1]河南中医药大学,河南郑州450046 [2]河南中医药大学第三附属医院,河南郑州450003

出　　处：《中医药学报》2023年第5期33-37,共5页Acta Chinese Medicine and Pharmacology

基　　金：国家自然科学基金青年基金项目(81804196);河南省省级科技研发计划联合基金(优势学科培育类)(222301420077);河南省高等学校青年骨干教师培养计划(2019GGJS108);河南中医药大学博士科研启动项目(RSBSJJ2019-16)。

摘　　要：目的:通过观察针刺和艾灸对顺铂(DDP)所致肝损伤小鼠肝脏中信号传导及转录激活蛋白3(STAT3)和细胞周期蛋白D1(CyclinD1)表达的变化,以及血清中谷氨酸脱氢酶(GLDH)、谷丙转氨酶(ALT)和谷草转氨酶(AST)活性变化,并结合小鼠肝脏超微病理,阐释针灸对DDP所致肝损伤小鼠的保护机制。方法:选用40只清洁级雄性KM小鼠,随机分为4组,空白组按照0.01 mL/g腹腔注射0.9%NaCl溶液,模型组、针刺组、艾灸组按照体质量腹腔注射同剂量DDP溶液,24 h后模型成功。治疗组配伍腧穴“大椎”“肝俞”“肾俞”“足三里”,分别给予针刺和艾灸治疗,其余2组陪同固定不治疗,1次/d,持续5 d。断食24 h后取材,生化检测血清中GLDH、ALT、AST活性,Western Blot测定肝脏中STAT3、CyclinD1蛋白表达量,电镜下观察肝脏超微病理变化。结果:与空白组比,模型组GLDH、ALT、AST活性升高,STAT3蛋白表达升高,CyclinD1蛋白表达降低,差异均有统计学意义(P<0.05)。与模型组比,针刺组和艾灸组GLDH、ALT、AST活性降低,STAT3蛋白表达降低,CyclinD1蛋白表达升高,差异均有统计学意义(P<0.05),针刺组与艾灸组比较,差异无统计学意义(P>0.05)。结论:针刺和艾灸可能通过调控肝脏中STAT3和CyclinD1蛋白的表达,促进肝脏细胞的增殖和分化,降低DDP所致肝损伤,对肝脏起到有效的保护作用。Objective:To elaborate the protective mechanism of acupuncture and moxibustion in improving liver injury by observing the protein expression changes of signal transduction and transcriptional activator protein 3(STAT3)and cyclin D1(CyclinD1)in liver of mice with cisplatin-diamminedichloro-platinum(DDP)induced liver injury,and the activity changes of glutamate dehydrogenase(GLDH),alanine aminotransferase(ALT)and aspartate aminotransferase(AST)in serum,in combination with ultramicropathology of liver in mice.Methods:40 male KM mice were randomly divided into the blank group,the model group,the acupuncture group and the moxibustion group.The blank group was intraperitoneally injected with 0.9%NaCl solution at the dose of 0.01 ml/g,the other three groups were intraperitoneally injected with the same dose of DDP solution according to body weight for modeling.After 24 h successful modeling,the acupoints of Dazhui(DU14),Ganshu(BL18),Shenshu(BL23)and Zusanli(ST36)were chosen for the intervention in the acupuncture group and in the moxibustion group,and the intervention was once a day for 5 days;meanwhile the mice in the blank group and in the model group were fixed without treatment.The activities of GLDH,ALT and AST in serum were detected by biochemical analysis.The protein expressions of STAT3 and CyclinD1 in liver were determined by Western Blot.The ultrastructural changes of liver were observed by electron microscopy.Results:Compared with those in the blank group,the activities of GLDH,ALT and AST were increased,the protein expression of STAT3 was increased,and the protein expression of CyclinD1 was decreased in the model group(P<0.05).Compared with those in the model group,the activities of GLDH,ALT and AST were significantly decreased,the protein expression of STAT3 was decreased,and the protein expression of CyclinD1 was increased in the acupuncture group and in the moxibustion group(P<0.05);in which there were no statistical differences between the acupuncture group and the moxibustion group(P>0.05).Conclusion:Ac

关 键 词：针灸 顺铂 肝损伤 信号传导及转录激活蛋白3 细胞周期蛋白D1 

分 类 号：R245[医药卫生—针灸推拿学]