Effects of Different Sample Parts and Sterilization Methods on Contamination and Survival Rates of Shoots with Buds in Chinese Chestnut  

作　　者：Jingjing DU Meng WANG Jing LIU Xuan WANG Haie ZHANG Jingzheng ZHANG 

机构地区：[1]College of Horticulture Science and Technology,Hebei Normal University of Science and Technology,Changli 066600,China [2]Engineering Research Center of Chestnut Industry Technology,Ministry of Education,Qinhuangdao 066004,China [3]Hebei Key Laboratory of Horticultural Germplasm Excavation and Innovative Utilization,Qinhuangdao 066004,China [4]Hebei Collaborative Innovation Center of Chestnut Industry,Qinhuangdao 066004,China

出　　处：《Agricultural Biotechnology》2023年第2期20-25,共6页农业生物技术（英文版）

基　　金：Supported by The Sub-project of Hebei Collaborative Innovation Center of Chestnut Industry(202202);The Sub-project of Research and Development Center of Horticultural Crop Breeding Application Technology of Hebei University(YF201404);Chestnut Science and Technology Backyard of Hebei Qinglong;China Rural Special Technology Association。

摘　　要：In this study,we investigated the preferable explant types and sterilization method for tissue culture in Chinese chestnut,in order to provide a technical support for the asexual reproduction of Chinese chestnut.The base,middle and apex parts of annual shoots with buds in Chinese chestnut were sampled and cut to 2-3 cm stem segments each with one bud,then sterilized orderly with different duration in 2%NaClO plus 0.1%HgCl_(2)solutions.The results indicated that the duration of 2%NaClO for 20 min+0.1%HgCl_(2)for 15 min exerted an effective disinfection property on the middle parts of annual shoots with buds,and achieved a contamination rate under 5%and a survival rate over 90%.

关 键 词：Chinese chestnut Sterilization method EXPLANT Contamination rate 

分 类 号：S664.2[农业科学—果树学]