Improving the Heat Resistance ofβ-1,4 Glucanase by Introducing Disulfide Bonds  

作　　者：Guodong WANG Junqing WANG 

机构地区：[1]Department of Biological Engineering,Qilu University of Technology,Jinan 250353,China [2]State Key Laboratory of Biobased Material and Green Papermaking(LBMP),Qilu University of Technology,Jinan 250353,China

出　　处：《Agricultural Biotechnology》2023年第2期32-37,共6页农业生物技术（英文版）

基　　金：Supported by the National Key Research and Development Plan of China(2019YFC1905902,2019YFC1905900);Key Research and Development Plan in Shandong Province(2020CXGC010603,2021ZDSYS10,2022CXGC020206);"Open Competition Mechanism"Project of Qilu University of Technology(Shandong Academy of Sciences)(2022JBZ01-06);Taishan Industry Leading Talent Program(tscy20180103);National Natural Science Foundation of China(31801527)。

摘　　要：Each possible pair of residues inβ-1,4 glucanase for disulfide formation was assessed using online websites,and four pairs L28C-S256C,Q41C-P278C,S122C-N163C and A184C-A215C were selected.Accordingly,four recombinant plasmids pET28a(+)EccslH28,pET28a(+)EccslH41,pET28a(+)EccslH122 and pET28a(+)EccslH184 were prepared and transformed into E.coli to express the recombinant enzymes.Then analysis on enzymatic properties showed that T50 of the recombinant enzymes was increased from 10 min for EccslHt2 to 90 min for EccslH28 and 40 min for EccslH41 at 70℃,while their optimum pH value and pH stability were not affected,which proved that the introduction of disulfide bond improved the thermal stability ofβ-1,4 glucanase.

关 键 词：β-1 4-Glucanase Disulfide bond Thermal stability Plasmid construction 

分 类 号：Q814[生物学—生物工程]