GRB7基因在5个猪群体内的多态性分析  

Polymorphism analysis of GRB7 gene in five porcine populations

作　　者：吴华莉[1,2] 张莺莺 黄济 王洪洋 曹建国 涂尾龙[1,2] 范春 谈永松[1,2] WU Huali;ZHANG Yingying;HUANG Ji;WANG Hongyang;CAO Jianguo;TU Weilong;FAN Chun;TAN Yongsong(Institute of Animal Husbandry and Veterinary Science,Shanghai Academy of Agricultural Sciences,Shanghai 201106,China;Shanghai Pig Breeding and Engineering Research Center,Shanghai 201302,China;Shanghai Laboratory Animal Research Center,Shanghai 201302,China)

机构地区：[1]上海市农业科学院畜牧兽医研究所,上海201106 [2]上海种猪工程技术研究中心,上海201302 [3]上海实验动物研究中心,上海201302

出　　处：《上海农业学报》2023年第2期71-75,共5页Acta Agriculturae Shanghai

基　　金：宁波市科技创新2025重大专项(2019B10023);上海市科技兴农项目[沪农科创字(2019)第3-2号];国家林业与草原局项目(野猪致害监测评估、种群调控、野猪死体检疫和无害化处理技术指南);上海市科技兴农项目[沪农科创字(2022)第1-1号]。

摘　　要：采用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)分析GRB7基因在杜洛克猪、长白猪、大白猪、梅山猪和申农猪群体内的多态性。结果表明:猪GRB7基因片段长度为626 bp,检测发现该片段的163 bp和243 bp处存在突变位点。163位点突变在杜洛克猪群体内CC基因型较多,在长白猪、大白猪和申农猪群体内CT基因型较多,在梅山猪群体内TT基因型较多。243位点突变在杜洛克猪、长白猪和大白猪群体内CT基因型较多,在申农猪和梅山猪群体内TT基因型较多。GRB7基因TTTT合并基因型在申农猪和梅山猪群体内为优势基因型,在杜洛克猪和长白猪群体内CCTC合并基因型频率较高,在大白猪群体内CTCT合并基因型频率较高。In order to investigate the polymorphism of GRB7 gene in Shanghai,we analyzed the polymorphism of GRB7 gene in Duroc,Landrace,Large white,Meishan and Shennong pig populations by PCRRFLP.The results indicated that the length of 626 bp fragment of GRB7 gene was amplified,there were mutation sites at 163 bp and 243 bp of this fragment.CC genotype at 163 site was dominant in Duroc pig populations,CT genotype was dominant in Landrace,Large White and Shennong pig populations,and TT genotype was dominant in Meishan pig population.CT genotype at 243 site was dominant in Duroc,Large White and Landrace populations,and TT genotype was dominant in Shennong and Meishan populations.TTTT combined genotype was dominant in Shennong and Meishan pig populations,CCTC combined genotype frequency was higher in Duroc and Landrace pig populations,and CTCT combined genotype frequency was higher in Large white pig populations.

关 键 词：生长因子受体结合蛋白7 限制性片段长度多态性 多态性分析 

分 类 号：S828[农业科学—畜牧学]