棕背䶄不同部位体毛红外光谱特征分析  

Comparison analysis of infrared spectrum characteristics of body hair in different parts of Myodes rufocanus

作　　者：杨文建 周思宇 苏航 万祥旭 金志民[1] YANG Wen-jian;ZHOU Si-yu;SU Hang;WANG Xiang-xu;JIN Zhi-min(College of Life Science and Technology,Mudanjiang Normal University,Mudanjiang 157011,Heilongjiang,China)

机构地区：[1]牡丹江师范学院生命科学与技术学院,黑龙江牡丹江157011

出　　处：《湖北农业科学》2023年第4期123-126,共4页Hubei Agricultural Sciences

基　　金：黑龙江省自然基金项目(LH2020C071;SS2021C006);牡丹江师范学院项目(2021H03)。

摘　　要：为了解棕背䶄(Myodes rufocanus)不同部位体毛的红外光谱特征,利用傅里叶变换红外光谱(FTIR)及其二阶导数谱(SD-IR)对棕背䶄的背毛和腹毛进行检测。结果表明,背毛和腹毛红外光谱在1560 cm^(-1)附近(酰胺Ⅱ带)、1637 cm^(-1)附近(酰胺Ⅰ带)均形成明显的吸收峰,蛋白质红外光谱中典型的酰胺基团吸收特征峰之一的酰胺Ⅳ带的O=C-N基团弯曲振动吸收峰在相同位置621、643 cm^(-1)出现,酰胺Ⅴ带的N-H基团的弯曲振动吸收峰均在678、781 cm^(-1)出现,但同时背毛在685 cm^(-1)处有酰胺Ⅴ带的N-H基团弯曲振动吸收峰,而腹毛则未观察到。在背毛和腹毛的二阶导数谱中,均在702、777、786、1646、1633、1654 cm^(-1)等处具有明显的基本一致的吸收峰。但同时背毛在726 cm^(-1)形成较小的吸收峰,而腹毛则未观察到,腹毛在1687~1694 cm^(-1)范围内有一个明显的单峰,背毛则有一个不明显的W形图谱。棕背䶄背毛和腹毛均能反映该物种的特征性成分。在600~800 cm^(-1)范围内酰胺基团表现较为明显,背毛和腹毛的红外图谱和二阶导数谱相似性极大。棕背䶄背毛和腹毛的红外光谱特征基本一致,但也存在一定的差异,尤其是二阶导数在600~800 cm^(-1)和1600~1800 cm^(-1)范围内的图谱能更好地反映出二者之间的差异。To understand the infrared spectrum characteristics of the body hair of different parts of Myodes rufocanus,Fourier trans⁃form infrared spectroscopy(FT-IR)and second derivative infrared spectroscopy(SD-IR)were used to analyze the dorsal and abdomi⁃nal hairs of the Myodes rufocanus.Results showed that the infrared spectrum of dorsal and abdominal hairs formed obvious absorption peaks around 1560 cm^(-1)(amideⅡband)and 1637 cm^(-1)(amideⅠband).The bending vibration absorption peak of O=C-N groups of the amideⅣband,one of the typical amide group absorption characteristic peaks in the protein infrared spectrum,appeared in the same position(621,643 cm^(-1)).The bending vibration absorption peaks of N-H groups in the amideⅤband all appeared in 678,781 cm^(-1),but at the same time,the back hair had the bending vibration absorption peaks of N-H groups in the amide side band in 685 cm^(-1),but the abdominal hair was not observed.In the second derivative spectra of the back hair and abdominal hair,the absorp⁃tion peaks in 702,777,786,1646,1633 and 1654 cm^(-1) were basically the same.However,the back hair had a smaller peak in 726 cm^(-1),while the abdominal hair had a distinct single peak in 1687~1694 cm^(-1),and the back hair had an indistinct W-shaped pat⁃tern in this position.The back hair and abdominal hair of Myodes rufocanus could reflect the characteristics of the species.In the range of 600~800 cm^(-1),the amide group was more obvious,and the infrared spectrum and second derivative spectrum of back and abdomi⁃nal hair were very similar.In addition,there were differences in the infrared spectrum of Myodes rufocanus back hair and abdominal hair,especially in the range of 600~800 cm-1 and 1600~1800 cm^(-1).

关 键 词：棕背䶄(Myodes rufocanus) 体毛 傅里叶变换红外光谱 二阶导数谱 

分 类 号：S764.5[农业科学—森林保护学] O657.33[农业科学—林学]