阴道内乳杆菌与粪肠球菌共培养生长状态及其上清液对阴道加德纳菌抑制作用的体外研究  

In vitro study on the growth state of intravaginal co-culture of Lactobacillus and Enterococcus faecalis and the inhibitory effect of supernatant on Gardnerella vaginalis

作　　者：张晓宇 孙茜 冯旸子 何渊慧 白会会 杜梦瑶 范琳媛 刘朝晖 Zhang Xiaoyu;Sun Qian;Feng Yangzi(Beijing Obstetrics and Gynecology Hospital,Capital Medical University,Beijing Maternal and Child Health Care Hospital,Beijing 100026;Capital Medical University,Beijing 100069)

机构地区：[1]首都医科大学附属北京妇产医院/北京妇幼保健院妇科,北京100026 [2]首都医科大学,北京100069 [3]首都医科大学附属北京同仁医院妇科,北京100730

出　　处：《现代妇产科进展》2023年第4期273-278,共6页Progress in Obstetrics and Gynecology

摘　　要：目的:研究阴道菌群中乳杆菌与粪肠球菌共培养后的生长特性,同时探究乳杆菌与粪肠球菌共培养上清液对阴道加德纳菌生物膜形成的影响。方法:选取临床诊断为BV患者的阴道分泌物标本中分离纯化鉴定的粪肠球菌和阴道加德纳菌,在妇科阴道微生态评价系统中诊断为菌群异常患者阴道分泌物标本中分离纯化鉴定的卷曲乳杆菌,观察乳杆菌与粪肠球菌共培养24、48、72h的生长情况及产乳酸能力。通过检测OD值观察阴道加德纳菌单独培养与分别加乳杆菌、粪肠球菌及其混合培养的上清液共培养24、48、72h后的生物膜形成情况。结果:乳杆菌与粪肠球菌混合培养后产L-乳酸比单独乳杆菌和单独粪肠球菌少,差异有统计学意义(P<0.05)。随着时间的增加,乳杆菌与粪肠球菌共培养后的菌量逐渐增多,但比粪肠球菌单独培养菌量多(P<0.05)。随着时间增加,乳杆菌与粪肠球菌共培养的成膜能力较乳杆菌、粪肠球菌单独培养的成膜能力好(P<0.05)。24h时乳杆菌上清液对加德纳菌成膜能力无明显抑制作用,而粪肠球菌上清液及乳杆菌与粪肠球菌共培养的上清液对加德纳菌生物膜形成有明显的抑制作用,差异有统计学意义(P<0.05);48h和72h时乳杆菌上清液、粪肠球菌上清液、乳杆菌与粪肠球菌共培养的上清液对加德纳菌的成膜能力均有很强的抑制作用,且共培养的上清液较单独培养的上清液抑制作用强,差异有统计学意义(P<0.05)。结论:乳杆菌与粪肠球菌共培养后在产乳酸方面存在拮抗作用,同时共培养后可相互促进生物膜的形成,而不促进细菌的生长。随着培养时间增加,乳杆菌与粪肠球菌共培养的上清液对阴道加德纳菌生物膜的抑制作用比单乳杆菌或单粪肠球菌上清液强,表明乳杆菌与粪肠球菌共培养后对加德纳菌生物膜形成的抑制有协同作用。Objective:To study the growth characteristics of Lactobacillus and Enterococcus faecalis co-cultured in vaginal flora,and to explore the effect of the supernatant of Lactobacillus and Enterococcus faecalis co-cultured on the formation of Gardnerella vaginalis biofilm.Methods:Enterococcus faecalis and Gardner vaginalis isolated,purified and identified from vaginal secretions of patients with clinical diagnosis of BV,and Lactobacillus curlicus isolated,purified and identified from vaginal secretions of patients with abnormal flora diagnosed in the gynecological vaginal microecology evaluation system.The growth and lactic acid production ability of Lactobacillus and Enterococcus faecalis co-cultured for 24h,48h and 72h were observed.Observe the biofilm formation of Gardnerella vaginalis after being cultured alone and co-cultured with the supernatant of Lactobacillus,Enterococcus faecalis and their mixed cultures for 24h,48h and 72h by detecting the OD value.Results:After mixed culture of Lactobacillus and Enterococcus faecalis,the production of L-lactic acid was less than that of Lactobacillus alone and Enterococcus faecalis alone(P<0.05).With the increase of time,the number of bacteria co-cultured with Lactobacillus and Enterococcus faecalis increased gradually,but more than that of Enterococcus faecalis alone(P<0.05).With the increase of time,the film forming ability of co-culture of Lactobacillus and Enterococcus faecalis was better than that of single culture of Lactobacillus and Enterococcus faecalis(P<0.05).At 24h,the supernatant of Lactobacillus had no significant inhibition on the membrane forming ability of Gardner bacteria,while the supernatant of Enterococcus faecalis and the supernatant of co-culture of Lactobacillus and Enterococcus faecalis had significant inhibition on the biofilm formation of Gardner bacteria(P<0.05).At 48h and 72h,the supernatant of Lactobacillus,Enterococcus faecalis,and the supernatant co-cultured with Lactobacillus and Enterococcus faecalis had a strong inhibitory effect on the m

关 键 词：阴道乳杆菌 粪肠球菌 阴道加德纳菌 共培养 益生菌 

分 类 号：R711.31[医药卫生—妇产科学]