三株鹅源坦布苏病毒的分离鉴定及遗传进化分析  

Isolation,Identification and Genetic Evolution Analysis of Three Strains of Goose-origin Tembusu Viruse

作　　者：童佳欣 王杰煌 朱婉君 涂军 张济培[1] 陈济铛[1] TONG Jiaxin;WANG Jiehuang;ZHU Wanjun;TU Jun;ZHANG Jipei;CHEN Jidang(Foshan University,Foshan,Guangdong 528225;Foshan Tianmu Biotechnology Co.,Ltd.,Foshan,Guangdong 528225;Guangdong Zhengdakang Biotechnology Co.,Ltd.,Foshan,Guangdong 528100)

机构地区：[1]佛山科学技术学院,广东佛山528225 [2]佛山天牧生物科技有限公司,广东佛山528225 [3]广东正大康生物科技股份有限公司,广东佛山528100

出　　处：《中国家禽》2023年第5期48-56,共9页China Poultry

基　　金：国家自然科学基金项目(31800141);广东省自然科学基金博士启动基金(2017A030310573);广东省乡村振兴战略专项基金(163-2019-XMZC-0009-02-0075)。

摘　　要：为了解广东地区鹅源坦布苏病毒流行情况,研究对自2020年6月至2021年6月从广东地区疑似坦布苏病毒(Tembusu virus,TMUV)感染鹅的组织病料进行TMUV分离鉴定。结果显示:共分离出3株鹅源TMUV,可感染BHK-21细胞并导致细胞病变;3株分离株核苷酸相似性为99.2%~99.7%,与Cluster2.1参考株基因同源性最高,为95.9%~99.3%,与Cluster2.2参考毒株同源性相对较低,为95.0%~96.4%;全基因组遗传进化分析和E蛋白氨基酸序列分析结果表明,3株鹅源分离株均位于Cluster2.1,其中Goose/GD/939/2021和Goose/GD/1025/2021 E蛋白氨基酸序列与其他毒株E蛋白相比多个位点存在差异。研究表明,从广东地区分离的3株鹅源TMUV均位于Cluster2.1,为进一步研究广东地区鹅源TMUV流行、进化及相关疾病的防控奠定基础。In order to understand the prevalence of goose-origin Tembusu virus(TMUV)in Guangdong province,tissue samples of suspected TMUV infected geese from June 2020 to June 2021 were uesd for isolation and identification of TMUV.The results showed that three strains of TMUV which could cause significantly cytopathic effect in BHK-21 cells were identified.The nucleotide homology among the three isolates were from 99.2%to 99.7%,the viral genome homology among three isolates and clus⁃ter 2.1 reference strains were from 95.9%to 99.3%,which was considered to be generally higher than that with cluster 2.2 refer⁃ence strains,which was from 95.0%to 96.4%.The results of phylogenetic analysis and E protein amino acid sequence analysis showed that the three goose-origin isolates were all clustered in cluster 2.1 branch.Comparing to the previously reported avian TMUV strains,multiple amino acid variations in E protein were noticed in Goose/GD/939/2021 and Goose/GD/1025/2021 of three isolates found in this study.It′s suggested that all of the three goose-origin strains of TMUV isolated in Guangdong located in cluster 2.1,which laid the foundation for the epidemic and evolution of TMUV and the prevention and control of associated dis⁃eases in Guangdong.

关 键 词：坦布苏病毒 鹅 BHK-21细胞 E基因 遗传进化 

分 类 号：S855.3[农业科学—临床兽医学]