海南新村湾泰来草内生细菌与内生固氮菌的多样性与群落结构研究  

The diversity and community structure of endophytic bacteria and endophytic nitrogen-fixing bacteria in Thalassia hemprichii of Xincun Bay in Hainan Province

作　　者：彭秋颖 凌娟[1,2,5] 孙英婷 田新朋 杨清松[1,2,5] 周卫国 周毅[3] 董俊德 PENG Qiuying;LING Juan;SUN Yingting;TIAN Xinpeng;YANG Qingsong;ZHOU Weiguo;ZHOU Yi;DONG Junde(South China Sea Institute of Oceanology,Chinese Academy of Sciences,CAS Key Laboratory of Tropical Marine Bio-resources and Ecology,Chinese Academy of Sciences,Guangdong Provincial Key Laboratory of Applied Marine Biology,Guangzhou 510301,China;Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou),Guangzhou 511458,China;CAS Key Laboratory of Marine Ecology and Environmental Sciences,Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China;Key Laboratory of Tropical Marine Biotechnology of Hainan Province,Sanya Institute of Oceanology,SCSIO,Tropical Marine Biological Research Station in Hainan,Chinese Academy of Sciences,Sanya 572000,China;Innovation Academy of South China Sea Ecology and Environmental Engineering,Chinese Academy of Sciences,Guangzhou 5114581,China;University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]中国科学院南海海洋研究所,中国科学院热带海洋生物资源与生态重点实验室,广东省应用海洋生物学重点实验室,广州510301 [2]南方海洋科学与工程广东省实验室(广州),广州511458 [3]中国科学院海洋研究所海洋生态与环境科学重点实验室,青岛266071 [4]海南省热带海洋生物技术重点实验室,三亚中科海洋研究院,中国科学院海南热带海洋生物实验站,三亚572000 [5]中国科学院南海生态环境工程创新研究院,广州511458 [6]中国科学院大学,北京100049

出　　处：《生态科学》2023年第3期17-29,共13页Ecological Science

基　　金：海南省自然科学基金(422QN440);南方海洋科学与工程广东省实验室(广州)人才团队引进重大专项(GML2019ZD0402);广东省科技计划项目(2020B1212060058,2021B1212050023);中国科学院南海生态环境工程创新研究院创新发展基金项目(ISEE2021ZD03);国家自然科学基金(41676163);广东省公益研究与能力建设专项资金(2015A020216016)。

摘　　要：内生菌对宿主植物的生长、发育、健康状态具有重要影响。通过构建克隆文库解析泰来草不同营养器官中内生细菌、内生固氮菌的群落组成和结构。从表面消毒的不同泰来草组织中提取DNA,基于16S rRNA和固氮基因nifH分别构建克隆文库,分析泰来草不同营养器官内生细菌和内生固氮菌的群落结构特征。新村湾泰来草共获得内生细菌分类单元(Operational Taxonomic Units,OTUs)162个,根、地下匍匐茎、叶分别含有72、45、52个OTUs,其中α-变形杆菌和γ-变形杆菌是优势类群,其相对丰度分别约为48.34%—62.72%和8.41%—33.21%。内生固氮菌OTUs 90个,根、地下匍匐茎、叶的OTUs数分别为59、19、27个,α-变形杆菌是优势类群,在根、地下匍匐茎和叶中所占的比例分别约为47.73%、48.99%和67.65%。泰来草不同组织部位内生细菌和内生固氮菌具有丰富的多样性,但是各个组织部位的优势菌存在差异,其中,根的内生细菌群落丰富度最高,叶的多样性最高;内生固氮菌群落的丰度和多样性最高的是根。另外,层级聚类分析和非度量多维尺度分析结果显示,泰来草根、地下匍匐茎和叶片三者的内生细菌、内生固氮菌群落结构均具有显著差异(R=1,P≤0.01)。本初步研究成果可为挖掘海草功能微生物提供新的视野,进一步将其应用于海草床的生态修复中。Endophyte play an important role in plant growth and development.We deployed the clone library approach to study the community composition of endophytic bacteria and nitrogen fixing bacteria in the Thalassia hemprichii of Xincun Bay.The total DNA was extracted from different surface-sterilized Thalassia hemprichii tissues,and clone libraries of 16S rRNA gene and nitrogen fixing gene nifH were constructed.A total of 162 different OTUs(Operational Taxonomic Units)of endophytic bacteria were obtained from 16S rRNA libraries.The OTUs obtained from root,rhizome and leaf were 72,45 and 52 respectively,classified to 9 phyla,17 classes,36 orders,62 families,108 genera.The dominant groups were Alphaproteobacteria(48.34%-62.72%),followed by Gammaproteobacteria(8.41%-33.21%).Besides,90 OTUs of endophytic nitrogen fixing bacteria were recovered from the established libraries.The OTUs of the root,rhizome and leaf were 59,19 and 27 respectively,belonging to 3 phyla,7 classes,13 orders,17 families,19 genera.Alphaproteobacteria dominated the community composition with an average relative abundance of 47.73%,48.99%and 67.65%in the root,rhizome and leaf,respectively.The results demonstrated a good diversity of endophytic bacteria and endophytic nitrogen fixing bacteria in the different tissues of Thalassia hemprichii.Moreover,the results also showed a different pattern of dominant bacterial groups in the various tissues.The endophytic bacterial community was highly abundant in root and the most diverse in leaf.Root had the highest abundance and diversity of endophytic nitrogen-fixing bacteria.In addition,cluster analysis and non-metric multidimensional scaling analysis showed that there were significant differences in the community structure of endophytic bacteria and nitrogen-fixing bacteria among root,rhizome and leaf(R=1,P≤0.01).These provided a theoretical reference for the application of endophytic bacteria in the ecological restoration of seagrass beds.

关 键 词：内生细菌 内生固氮菌 多样性 泰来草 

分 类 号：P76[天文地球—海洋科学]