基于漆酶催化角蛋白头发纤维生物修复染色  

Bioremediation dyeing of keratin hair fibers with dopamine hydrochloride catalyzed by laccase

作　　者：贾维妮[1,2] 王涛 鲍杰 梁志结 王海峰 JIA Weini;WANG Tao;BAO Jie;LIANG Zhijie;WANG Haifeng(College of Textile and Garment,Nantong University,Nantong,Jiangsu 226019,China;National Manufacturing Innovation Center of Advanced Dyeing and Finishing Technology,Taian,Shandong 271000,China)

机构地区：[1]南通大学纺织服装学院,江苏南通226019 [2]国家先进印染技术创新中心,山东泰安271000

出　　处：《纺织学报》2023年第4期108-114,共7页Journal of Textile Research

基　　金：南通市科技计划项目(JC2020126);江苏省高等学校大学生创新实验计划项目(202010304057Z);南通大学横向合作项目(19ZH590,19ZH591,20ZH104);江苏省产学研合作项目(BY2020556)。

摘　　要：针对目前常规染发过程中氧化剂及染发剂对角蛋白头发纤维的损伤及易对人产生过敏等问题,采用漆酶催化盐酸多巴胺聚合生色并对角蛋白头发纤维进行原位染色,以达到生态安全、环保健康、生物修复、染色牢固的角蛋白头发纤维染黑效果。借助测色配色仪、万能材料试验机、扫描电子显微镜、傅里叶变换红外光谱仪和纺织品防紫外性能测试仪等对染色角蛋白头发纤维的结构和性能进行了表征。结果表明:原位染色角蛋白头发纤维表面色深值为23.9,表观颜色色相为黑色,防紫外线性能也得到提高,紫外线防护系数为100+,染色后角蛋白头发纤维断裂强力提高7.8%;漆酶催化盐酸多巴胺聚合生成聚多巴胺色素,且附着于角蛋白头发纤维表面,与角蛋白头发纤维结合牢固,染色角蛋白头发纤维的干、湿摩擦色牢度及耐洗变色牢度分别达到4级、3~4级及4~5级。Objective In order to solve the problems of keratin hair fibers damage and allergy caused by the oxidant and keratin hair colorants in the current routine of keratin hair fibers dyeing process,the keratin hair fibers were dyed in situ by laccase catalyzed polymerization of dopamine hydrochloride to achieve blackening effect,accompanied by ecological restoration,health and fastness features.Method The in-situ dyeing process was performed in one step with 2.5 g/L dopamine hydrochloride,0.9 U/mL laccase at 50℃for 4 h.Factors,such as reaction time,temperature and laccase concentration,were investigated on the influence on determining the binding of pigment molecule to keratin hair fibers.Besides,the structure and properties of the dyed keratin hair fibers were characterized and analyzed by color parameter,single fiber strength,Fourier-transform infrared spectroscopy,scanning electron microscope,anti-UV and color fastness test.Results To understand the effect of pre-bleaching on keratin hair fibers coloring,the untreated natural white keratin hair fibers and white keratin hair fibers pretreated with hydrogen peroxide were dyed(Fig.5,Fig.6).After the pre-processing,more binding points were exposed to facilitate the penetration of the pigment formed in the in-situ dyeing process into the keratin hair fibers,reaching a darker color and good fastness.The results of SEM and K/S value as well as color fastness showed that the dyeing process was more suitable to the pretreated white keratin hair fibers,owing to the weaken cuticle layer of pretreated white keratin hair fibers.After dyeing,the cuticle layer of the keratin hair fibers was not clear,attributing to the uniform and dense polydopamine coating formed by a reaction catalyzed by laccase covering on the surface of the keratin hair fibers(Fig.7).Comparing to the IR spectra of keratin hair fibers before dyeing(Fig.8),fibers after dyeing showed a broad peak at 3273 cm-1,indicating that there were polydopamine pigments on the surface of keratin hair fibers.Peaks at 1634

关 键 词：角蛋白头发纤维 染色 漆酶 催化 盐酸多巴胺 修复 

分 类 号：TS195[轻工技术与工程—纺织化学与染整工程]