Virome diversity of ticks feeding on domestic mammals in China  

作　　者：Zijun Yang Hao Wang Shixing Yang Xiaochun Wang Quan Shen Likai Ji Jian Zeng Wen Zhang Haiyan Gong Tongling Shan 

机构地区：[1]Shanghai Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Shanghai,200241,China [2]Department of Microbiology,School of Medicine,Jiangsu University,Zhenjiang,212013,China [3]Department of Clinical Laboratory,Huai'an Hospital,Xuzhou Medical University,Huai'an,223002,China [4]Center of Clinical Laboratory,The First Affiliated Hospital of Soochow University,Suzhou,215006,China

出　　处：《Virologica Sinica》2023年第2期208-221,共14页中国病毒学（英文版）

基　　金：funded by the National Key Research and Development Programs of China(No.2022YFC2603800);Jiangsu Provincial Key Research and Development Projects(No.BE2017693).

摘　　要：Ticks are considered the second most common pathogen vectors transmitting a broad range of vital human and veterinary viruses.From 2017 to 2018,640 ticks were collected in eight different provinces in central and western China.Six species were detected,including H.longicornis,De.everestianus,Rh.microplus,Rh.turanicus,Rh.sanguineous,and Hy.asiaticum.Sixty-four viral metagenomic libraries were constructed on the MiSeq Illumina platform,resulting in 13.44 G(5.88×10^(7))of 250-bp-end reads,in which 2,437,941 are viral reads.We found 27 nearly complete genome sequences,including 16 genome sequences encoding entire protein-coding regions(lack of 30 or 50 end non-coding regions)and complete viral genomes,distributed in the arboviral family(Chuviridae,Rhabdoviridae,Nairoviridae,Phenuiviridae,Flaviviridae,Iflaviridae)as well as Parvoviridae and Polyomaviridae that cause disease in mammals and even humans.In addition,13 virus sequences found in Chuviridae,Nairoviridae,Flaviviridae,Iflaviridae,Hepeviridae,Parvoviridae,and Polyomaviridae were identified as belonging to a new virus species in the identified viral genera.Besides,an epidemiological survey shows a high prevalence(9.38%and 15.63%)of two viruses(Ovine Copiparvovirus and Bovine parvovirus 2)in the tick cohort.

关 键 词：Central and western China Viral metagenomes Tick-borne virus Phylogenetic analysis Domestic mammals Tick virome 

分 类 号：S852.65[农业科学—基础兽医学]