马鬃蛇石油醚提取物通过调控Caspase-1对动脉性勃起功能障碍大鼠勃起功能、生育功能的影响  

Study on Erectile Function and Reproductive Function of Rats with Arterial Erectile Dysfunction by Regulating Caspase-1 with Horsehair Snake Petroleum Ether Extract(CVPEE)

作　　者：王永军[1] 代晓微 严福军 WANG Yongjun;DAI Xiaowei;YAN Fujun(The Second Hospital of Shaoxing,Shaoxing,Zhejiang 312000;The Second Hospital of Jilin University,Changchun,Jilin 130041;Maternal and Child Health Hospital of Ruichang,Jiujiang,Jiangxi 332200)

机构地区：[1]绍兴第二医院,浙江绍兴312000 [2]吉林大学第二医院,吉林长春130041 [3]江西瑞昌市妇幼保健院,江西九江332200

出　　处：《中国中医药科技》2023年第3期447-451,共5页Chinese Journal of Traditional Medical Science and Technology

摘　　要：目的:探究马鬃蛇石油醚提取物(CVPEE)通过调控半胱氨酸的天冬氨酸蛋白水解酶(Caspase-1)对动脉性勃起功能障碍大鼠勃起功能及生育功能的影响。方法:将50只大鼠分为假手术组、模型组、提取物低剂量组(2 g/kg)和提取物高剂量组(4 g/kg)、阳性组(12 mg/kg万艾可),连续给药21 d;检测大鼠勃起功能[阴茎勃起次数、阴茎海绵体压力(ICP)]、生育功能(睾丸质量和指数、附睾精子数量、存活率)、阴茎海绵体细胞凋亡(TUNEL法)及组织中蛋白(Bax、Bcl-2和Caspase-1)表达。结果:与假手术组相比,模型组大鼠勃起次数和ICP值显著降低,睾丸和附睾质量及指数、精子密度和精子存活率均显著降低(P<0.05);提取物低剂量组和提取物高剂量组大鼠勃起次数和ICP值、睾丸和附睾质量及指数、精子密度和精子存活率均明显高于模型组(P<0.05)。模型组大鼠阴茎海绵体细胞凋亡率和Bax、Caspase-1蛋白表达高于假手术组,Bcl-2蛋白表达低于假手术组(P<0.05);提取物低剂量组和提取物高剂量组大鼠阴茎海绵体细胞凋亡率和Bax、Caspase-1蛋白表达低于模型组,Bcl-2蛋白表达高于模型组(P<0.05)。结论:马鬃蛇石油醚提取物对动脉性勃起功能障碍大鼠勃起功能和生育功能有明显的改善作用,可能和抑制阴茎海绵体细胞凋亡和Caspase-1蛋白表达有关。Objective:To explore the effect of Horsemane snake(Calotes Versicolore)Petroleum Ether Extract(CVPEE)on erectile function and reproductive function of rats with arterial erectile dysfunction by regulating Caspase-1 of cysteine.Methods:50 rats were divided into sham operation group,model group low-dose extract group(2 g/kg CVPEE),high-dose extract group(4 g/kg CVPEE),positive group(12 mg/kg Viagra).The erectile function[number of penile erections,penile cavernous pressure(ICP)],reproductive function(testicular mass and index,epididymal sperm number,survival rate),penile cavernous cell apoptosis(TUNEL method)and protein expressions in tissues(Bax,Bcl-2 and Caspase-1)were detected.Results:Compared with the sham operation group,the number of erections and ICP value of rats in the model group were significantly decreased,and the weight and index of testis and epididymis,sperm density and sperm survival rate were significantly decreased(P<0.05);The number of erections,ICP value,weight and index of testis and epididymis,sperm density and sperm survival rate of rats in the low-dose and high-dose CVPEE groups were significantly higher than those in the model group(P<0.05).The apoptosis rate of penile cavernous cells and the expressions of Bax and Caspase-1 protein in model group were higher than those in sham operation group,while the expression of Bcl-2 protein was lower than that in sham operation group(P<0.05);The apoptosis rate and the expressions of Bax and Caspase-1 protein in the corpus cavernosum of rats in the low-dose and high-dose CVPEE groups were lower than those in the model group,while the expression of Bcl-2 protein was higher than that in the model group(P<0.05).Conclusion:CVPEE can significantly improve the erectile function and reproductive function of rats with arterial erectile dysfunction,which may be related to inhibiting the apoptosis of penile cavernous cells and the expression of Caspase-1 protein.

关 键 词：马鬃蛇石油醚提取物 动脉性勃起功能障碍 勃起功能 生育功能 细胞凋亡 BAX Bcl-2 CASPASE-1 大鼠 

分 类 号：R285.5[医药卫生—中药学]