Bio-inspired dual-adhesive particles from microfluidic electrospray for bone regeneration  

作　　者：Lei Yang Xiaocheng Wang Yunru Yu Luoran Shang Wei Xu Yuanjin Zhao 

机构地区：[1]Department of Rheumatology and Immunology,Nanjing Drum Tower Hospital,School of Biological Science and Medical Engineering,Southeast University,Nanjing 210096,China [2]Oujiang Laboratory(Zhejiang Lab for Regenerative Medicine,Vision and Brain Health),Wenzhou Institute,University of Chinese Academy of Sciences,Wenzhou 325001,China [3]Shanghai Xuhui Central Hospital,Zhongshan-Xuhui Hospital,and the Shanghai Key Laboratory of Medical Epigenetics,the International Colaboratory of Medical Epigenetics and Metabolism(Ministry of Science and Technology),Institutes of Biomedical Sciences,Fudan University,Shanghai 200032,China [4]Department of Orthopedics,Tongren Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200336,China

出　　处：《Nano Research》2023年第4期5292-5299,共8页纳米研究（英文版）

基　　金：supported by the National Key Research and Development Program of China(No.2020YFA0908200);the National Natural Science Foundation of China(No.82202670);the Guangdong Basic and Applied Basic Research Foundation(No.2021B1515120054);the Shenzhen Fundamental Research Program(Nos.JCYJ20190813152616459 and JCYJ20210324133214038);the Startup Fund of Wenzhou Institute,University of Chinese Academy of Sciences(No.WIUCASQD2022001).

摘　　要：Bioadhesive hydrogels have demonstrated great potential in bone regeneration.However,the relatively simple adhesion mechanism and lack of intricate structural design restrict their further applications.Herein,inspired by multiple adhesion mechanisms of pollen particles and marine mussels,we present a novel type of dual-adhesive hydrogel particles fabricated from microfluidic electrospray for bone regeneration.As the particles are rapidly solidified via liquid nitrogen-assisted cryogelation,they exhibit pollen-mimicking hierarchical porous morphology and gain structure-related adhesion.Besides,the particles are further coated by polydopamine(PDA)to achieve molecular-level adhesion especially to physiological wet surfaces of bone issues.Benefiting from such dual-adhesion mechanisms,the particles can strongly adhere to bone tissue defects,and function as porous scaffolds.Moreover,the dual-adhesive particles can serve as effective vehicles to release key growth factors more than two weeks.In vitro experiments showed that the growth factors-loaden particles have excellent biocompatibility and more significantly promote angiogenesis(~2-fold)and osteogenic differentiation(~3-fold)than control.In vivo experiments indicated that the dual-adhesive particles could significantly enhance bone regeneration(~4-fold)than control by coupling osteogenesis and angiogenesis effects.Based on these features,the bio-inspired dual-adhesive particles have great potentials for bone repair and wound healing applications.

关 键 词：MICROFLUIDICS PARTICLES growth factors ADHESION bone regeneration 

分 类 号：R318[医药卫生—生物医学工程]