High-density single antibody electrochemical nanoarrays  

作　　者：Khalil Chennit Yannick Coffinier Shuo Li Nicolas Clément Agnès Anne Arnaud Chovin Christophe Demaille 

机构地区：[1]UniversitéParis Cité,CNRS,Laboratoire d’Electrochimie Moléculaire,Paris F-75013,France [2]Institute of Electronics,Microelectronics and Nanotechnology,CNRS,University of Lille,Avenue Poincaré,BP60069,Villeneuve d’Ascq 59652,France [3]IIS,LIMMS/CNRS-IIS UMI2820,The University of Tokyo,4-6-1 Komaba,Meguro-ku,Tokyo 153-8505,Japan

出　　处：《Nano Research》2023年第4期5412-5418,共7页纳米研究（英文版）

摘　　要：The fabrication and electrochemical interrogation of very high density single-antibody nanoarrays is reported.Gold nanodots,15 nm in diameter,arranged in large(cm2)square arrays with a pitch of 200 nm,are used as carriers for primary antibodies(immunoglobulin G(IgG)),further recognized by secondary redox-labeled detection antibodies.Ensemble scale interrogation of the antibody array by cyclic voltammetry,and nanoscale interrogation of individual nanodots by mediator tethered atomic forcescanning electrochemical microscopy(Mt/AFM-SECM),enable the occupancy of nanodots by single antibody molecules to be demonstrated.Experiments involving the competitive adsorption of antibodies of different species onto the nanodots evidence the possibility of using single-antibody nanoarrays for digital electrochemical immunoassays.

关 键 词：single entity electrochemistry electrochemical digital immunoassays atomic force-scanning electrochemical microscopy(AFM-SECM) 

分 类 号：O64[理学—物理化学]