Plasmonic polydopamine-modified TiO_(2)nanotube substrates for surface-assisted laser desorption/ionization mass spectrometry imaging  

作　　者：Dong Chen Mingyi Du Yudi Huang Yizhu Xu Yingying Chen Lianlian Ma Qingrong Xie Xinhai Zhu Zilong Chen Hanhong Xu Xinzhou Wu Zhibin Yin 

机构地区：[1]State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources and Key Laboratory of Natural Pesticide and Chemical Biology of the Ministry of Education,South China Agricultural University,Guangzhou 510642,China [2]Agro-biological Gene Research Center,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China [3]Instrumental Analysis and Research Center,Sun Yat-sen University,Guangzhou 510275,China

出　　处：《Nano Research》2023年第2期3028-3039,共12页纳米研究（英文版）

基　　金：the National Natural Science Foundation of China(Nos.31901911 and 21904142);the Natural Science Foundation of Guangdong Province(Nos.2019A1515011521 and 2022A1515011385);supported by the Young Talent Support Project of Guangzhou Association for Science and Technology(No.QT20220101031).

摘　　要：Mass spectrometry imaging(MSI)has made the spatio-chemical characterization of a broad range of small-molecule metabolites within biological tissues possible.However,available matrix-assisted laser desorption/ionization mass spectrometry(MALDI-MS)suffers from severe background interferences in low-mass ranges and inhomogeneous matrix deposition.Thus,surface-assisted LDI-MS(SALDI-MS)has been an attractive alternative for high-sensitivity detection and imaging of small biomolecules.In this study,we construct a new composite substrate,hydrophobic polydopamine(hPDA)-modified TiO_(2)nanotube(TDNT)coated with plasmonic gold nanoparticle(AuNP-hPDA-TDNT),as a dual-polarity SALDI substrate using an easy and cost-effective fabrication approach.Benefitting from the synergistic effects of TDNT semiconductor and plasmonic PDA modification,this SALDI substrate exhibits superior performance for dual-polarity detection of a vast diversity of small molecules.Highly reduced background interferences,lower detection limits,and spot-to-spot repeatability can be achieved using AuNP-hPDA-TDNT substrates.Due to its unique imprinting performance,various metabolites and lipids can be visualized within jatropha integerrima petals,ginkgo leaves,strawberry fruits,and latent fingerprints.More valuably,the universality of this matrix-free substrate is demonstrated for mapping spatial distribution of lipids within mouse brain tissue sections.Considered together,this AuNP-hPDA-TDNT material is expected to be a promising SALDI substrate in various fields,especially in nanomaterial development and life sciences.

关 键 词：TiO_(2)nanotube surface-assisted laser desorption/ionization(SALDI) mass spectrometry imaging latent fingerprint tissue imaging 

分 类 号：TB383[一般工业技术—材料科学与工程]