Thermal-annealing-regulated plasmonic enhanced fluorescence platform enables accurate detection of antigen/antibody against infectious diseases  

作　　者：Ying Yue Jingjie Nan Yuanyuan Che Hongqin Xu Weihong Sun Feiran Zhang Lei Wang Wei Xu Junqi Niu Shoujun Zhu Junhu Zhang Bai Yang 

机构地区：[1]State Key Laboratory of Supramolecular Structure and Materials,College of Chemistry,Jilin University,Changchun 130012,China [2]Joint Laboratory of Opto-Functional Theranostics in Medicine and Chemistry,The First Hospital of Jilin University,Changchun 130021,China [3]Department of Laboratory Medicine,The First Hospital of Jilin University,Changchun 130021,China [4]Department of Hepatology,Center of Infectious Diseases and Pathogen Biology,The First Hospital of Jilin University,Changchun 130021,China

出　　处：《Nano Research》2023年第2期3215-3223,共9页纳米研究（英文版）

基　　金：supported by the National Natural Science Foundation of China(Nos.21975098 and 22275071);the program for JLU Science and Technology Innovative Research Team(No.2017TD-06);the opening funds of State Key Laboratory of Applied Optics,Changchun Institute of Optics,Fine Mechanics and Physics,Chinese Academy of Science,and the China Postdoctoral Science Foundation(Nos.2020TQ0119 and 2020M681046).

摘　　要：Plasmonic enhanced fluorescence(PEF)technology is a powerful strategy to improve the sensitivity of immunofluorescence microarrays(IFMA),however,current approaches to constructing PEF platforms are either expensive/time-consuming or reliant on specialized instruments.Here,we develop a completely alternative approach relying on a two-step protocol that includes the self-assembly of gold nanoparticles(GNPs)at the water–oil interface and subsequent annealing-assisted regulation of gold nanogap.Our optimized thermal-annealing GNPs(TA-GNP)platform generates adequate hot spots,and thus produces high-density electromagnetic coupling,eventually enabling 240-fold fluorescence enhancement of probed dyes in the near-infrared region.For clinical detection of human samples,TA-GNP provides super-high sensitivity and low detection limits for both hepatitis B surface antigen and SARS-CoV-2 binding antibody,coupled with a much-improved detection dynamic range up to six orders of magnitude.With fast detection,high sensitivity,and low detection limit,TA-GNP could not only substantially improve the outcomes of IFMA-based precision medicine but also find applications in fields of proteomic research and clinical pathology.

关 键 词：plasmonic enhanced fluorescence electromagnetic coupling SARS-CoV-2 antibodies hepatitis B surface antigen immunofluorescence microarrays 

分 类 号：O657.3[理学—分析化学]