Amoeba-inspired magnetic microgel assembly assisted by engineered dextran-binding protein for vaccination against lifethreatening systemic infection  

作　　者：Shuo Liu Yan Zhao Linpei Guo Qilin Yu 

机构地区：[1]Key Laboratory of Molecular Microbiology and Technology,Ministry of Education,Department of Microbiology,College of Life Sciences,Nankai University,Tianjin 300071,China [2]Research Center for Infectious Diseases,Nankai University,Tianjin 300350,China [3]College of Environmental Science and Engineering,Tianjin Key Laboratory of Environmental Remediation and Pollution Control,Nankai University,Tianjin 300350,China [4]Department of Urology,The Affiliated Wuxi No.2 People’s Hospital of Nanjing Medical University,Wuxi 214002,China

出　　处：《Nano Research》2023年第1期938-950,共13页纳米研究（英文版）

基　　金：This work was supported by the National Natural Science Foundation of China(Nos.3217010793 and 31870139);Tianjin Synthetic Biotechnology Innovation Capacity Improvement Project(No.TSBICIP-KJGG-006);the Natural Science Foundation of Tianjin(No.19JCZDJC33800);the Fundamental Research Funds for the Central Universities.

摘　　要：Vaccination is critical for population protection from pathogenic infections.However,its efficiency is frequently compromised by a failure of antigen retention and presentation.Herein,we designed a dextran-binding protein DexBP,which is composed of the carbohydrate-binding domains of Trichoderma reesei cellobiohydrolases Cel6A and Cel7A,together with the sequence of the fluorescent protein mCherry.DexBP was further prepared by engineered Escherichia coli cells and grafted to magnetic nanoparticles.The magnetic nanoparticles were integrated with a dextran/poly(vinyl alcohol)framework and a reactive oxygen species-responsive linker,obtaining magnetic polymeric microgels for carrying pathogen antigen.Similar to amoeba aggregation,the microgels self-assembled to form aggregates and further induced dendritic cell aggregation.This step-by-step assembly retained antigens at lymph nodes,promoted antigen presentation,stimulated humoral immunity,and protected the mice from lifethreatening systemic infections.This study developed a magnetic microgel-assembling platform for dynamically regulating immune response during protection of the body from dangerous infections.

关 键 词：magnetic nanoparticle engineered protein DEXTRAN MICROGEL VACCINATION systemic infection 

分 类 号：O62[理学—有机化学]