A green light-enhanced cytosolic protein delivery platform based on BODIPY-protein interactions  

作　　者：Yang Zhou Yifan Gao Li Pang Weirong Kang Kwan Man Weiping Wang 

机构地区：[1]State Key Laboratory of Pharmaceutical Biotechnology,The University of Hong Kong,Hong Kong,China [2]Department of Pharmacology and Pharmacy,Li Ka Shing Faculty of Medicine,The University of Hong Kong,Hong Kong,China [3]Dr.Li Dak-Sum Research Centre,The University of Hong Kong,Hong Kong,China [4]Department of Surgery,School of Clinical Medicine,HKU-SZH&LKS Faculty of Medicine,The University of Hong Kong,Hong Kong,China

出　　处：《Nano Research》2023年第1期1042-1051,共10页纳米研究（英文版）

基　　金：This work was supported by the Research Grants Council of Hong Kong(Early Career Scheme,No.27115220);Ming Wai Lau Centre for Reparative Medicine Associate Member Program.

摘　　要：Development of cytosolic protein delivery platforms brings new possibilities for various incurable diseases.Strategies based on polymer/protein self-assembly have shown their potential in protein delivery.However,versatile photocontrolled platforms based on self-assembly for protein delivery are seldom reported.Herein,we report a boron-dipyrromethene(BODIPY)-modified polyamidoamine(PAMAM)with excellent photo-controllability and efficiency for the cytosolic delivery of various proteins.High serum stability was achieved by coating hyaluronic acid and human serum albumin on the surface of BODIPY-modified PAMAM/protein nanoparticles.The nanoparticles under green light irradiation allowed efficient intracellular delivery of multiple cargo proteins with different charges and molecular weights and promoted endosome escape.The study provides valuable guidance for the development of BODIPY derivative-based protein delivery systems and advances the research in intracellular protein delivery.

关 键 词：photoresponsive nanoparticles intracellular delivery protein delivery polyamidoamine(PAMAM) boron-dipyrromethene(BODIPY) 

分 类 号：TB383[一般工业技术—材料科学与工程]