Bispecific aptamer-initiated 3D DNA nanomotor biosensor powered by DNAzyme and entropy-driven circuit for sensitive and specificity detection of lysozyme  

作　　者：Shuo Qi Yuhan Sun Xiaoze Dong Imran Mahmood Khan Yan Lv Yin Zhang Nuo Duan Shijia Wu Zhouping Wang 

机构地区：[1]State Key Laboratory of Food Science and Technology,International Joint Laboratory on Food Safety,Jiangnan University,Wuxi 214122,China [2]School of Food Science and Technology,Jiangnan University,Wuxi 214122,China [3]Key Laboratory of Meat Processing of Sichuan,Chengdu University,Chengdu 610106,China [4]National Engineering Research Center for Functional Food,Jiangnan University,Wuxi 214122,China [5]Collaborative Inno vation Center of Food Safety and Quality Control in Jiangsu Province,Jiangnan University,Wuxi 214122,China

出　　处：《Nano Research》2023年第1期1286-1295,共10页纳米研究（英文版）

基　　金：This work was partly funded by the National Natural Science Foundation of China(Nos.31871881 and 31871721);the National First-class Discipline Program of Food Science and Technology(No.JUFSTR20180303);the National High-Level Personnel of Special Support Program(No.W03020371).

摘　　要：Dynamic DNA nanodevices have gained tremendous attention due to their extraordinary inherent functionality and advantages,however,dynamic DNA nanodevices-based biosensors are still challenging due to their high reliance on proteases and limited amplification capabilities.Herein,exploiting bispecific aptamer as initiators for the first time,we developed a three-dimensional(3D)DNA nanomotor biosensor powered by DNAzyme and entropy-driven circuit for sensitive and specific detection of lysozyme,in which walking and rolling strategies are efficiently integrated to achieve excellent signal amplification capability.Benefiting from the high selectivity of bispecific aptamer,the 3D DNA nanomotor biosensor can respond to lysozyme with high specificity and operate at high speed to release signals.The whole process is independent of protease,avoiding the influence of adverse environment on the operation stability.Under optimal conditions,it can achieve a limit of detection as low as 0.01 pg/mL with an excellent linear range of 0.05 pg/mL–500 ng/mL for lysozyme.Furthermore,the proposed strategy revealed high accuracy in the analysis of real samples,indicating a great potential for the application of nanomotor biosensors to the detection of non-nucleic acid targets.

关 键 词：bispecific aptamer DNA rolling DNA walking amplification SPECIFICITY 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置]