木贼麻黄茎木脂素成分及其抗哮喘活性  

Lignans from the stems of Ephedra equisetina and their anti-asthmatic activities

作　　者：朱登辉 张靖柯 李孟[1,2] 刘娟娟 魏俊俊 冯卫生 郑晓珂[1,2] ZHU Deng-hui;ZHANG Jing-ke;LI Meng;LIU Juan-juan;WEI Jun-jun;FENG Wei-sheng;ZHENG Xiao-ke(College of Pharmacy,Henan University of Chinese Medicine,Zhengzhou 450046,China;Engineering and Technology Center for Chinese Medicine Development of Henan Province,Zhengzhou 450046,China)

机构地区：[1]河南中医药大学药学院,河南郑州450046 [2]河南省中药开发工程技术中心,河南郑州450046

出　　处：《中成药》2023年第5期1513-1520,共8页Chinese Traditional Patent Medicine

基　　金：国家重点研发计划-中医药现代化研究项目(2019YFC1708802);河南省重大科技专项项目(171100310500)。

摘　　要：目的研究木贼麻黄Ephedra equisetina Bunge.茎中木脂素,并考察其抗哮喘活性。方法木贼麻黄70%乙醇提取物采用硅胶柱、Sephadex LH-20、HPLC进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。采用底物显色法检测C48/80诱导的大鼠嗜碱性白血病细胞(RBL-2H3)的β-氨基己糖苷酶释放率。结果从中分离得到22个化合物。化合物1能抑制β-Hex释放,改善RBL-2H3脱颗粒现象。结论化合物1~22为首次从该植物中分离得到,其中化合物1具有潜在的抗哮喘活性。AIM To study the lignans from the stems of Ephedra equisetina Bunge.and to investigate their anti-asthmatic activities.METHODS The 70%ethanol extract from E.equisetina was isolated and purified by silica gel colum,Sephadex LH-20 and HPLC chromatographic method,then the structures of obtained compounds were identified by physicochemical properties and spectral data.The anti-asthmatic activities of lignans were evaluated by releasingβ-Hex in C48/80-induced RBL-2H3 cells with chromogenic substrate method.RESULTS Twenty-two compounds were isolated and identified.Compound 1 could inhibitβ-Hex release and improve the degranulation of RBL-2H3.CONCLUSION Compounds 1-22 are first isolated from this plant.Compound 1 possesses potentially anti-asthmatic activities.

关 键 词：木贼麻黄 茎 木脂素 抗哮喘活性 分离鉴定 

分 类 号：R284.1[医药卫生—中药学]