朱砂七化学成分及其体外细胞毒性、抗病毒活性研究  

doi:10.3969/j.issn.1001-1528.2023.05.025 Chemical constituents from Fallopia multiflora var.ciliinervis and their in vitro cytotoxic,anti-viral activities

作　　者：王冯瑞 王薇[1] 刘辰旺 但林蔚 李玉泽[1] 姜祎 张化为[1] 张东东[1] 宋小妹[1] WANG Feng-rui;WANG Wei;LIU Chen-wang;DAN Lin-wei;LI Yu-ze;JIANG Yi;ZHANG Hua-wei;ZHANG Dong-dong;SONG Xiao-mei(School of Pharmacy,Shaanxi University of Chinese Medicine,Xianyang 712046,China)

机构地区：[1]陕西中医药大学药学院,陕西咸阳712046

出　　处：《中成药》2023年第5期1537-1543,共7页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金项目(82104368);陕西省科技厅重点研发计划项目(2022SF-254);陕西中医药大学学科创新团队项目(2019-YL12)。

摘　　要：目的研究朱砂七Fallopia multiflora var.ciliinervis(Nakai)Yonekura&H.Ohashi化学成分及其体外细胞毒性、抗病毒活性。方法朱砂七75%乙醇提取物采用硅胶柱层析、半制备HPLC进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。采用MTT法测定体外细胞毒活性,CCK-8法测定体外抗病毒活性。结果从中分离得到20个化合物,分别鉴定为3-acetyl-4,5-dimethoxy-benzoic acid(1)、没食子酸甲酯(2)、没食子酸(3)、对羟基苯甲醛(4)、原儿茶酸(5)、3,4-二羟基苯甲醛(6)、丁香酸(7)、4-羟基-3-甲氧基苯甲酸(8)、p-hydroxyphenethyl-O-β-D-glucoside(9)、2-(4-hydroxy-3-methoxyphenyl)-ethyl-O-β-D-glucoside(10)、反式-白藜芦醇(11)、顺式-白藜芦醇(12)、白藜芦醇-3-O-β-D-(2′-O-反式-肉桂酰基)-葡萄糖苷(13)、白藜芦醇-3-O-β-D-(2′-O-顺式-肉桂酰基)-葡萄糖苷(14)、白藜芦醇-3-O-β-D-葡萄糖苷(15)、N-反式阿魏酰-3-甲氧基酪胺(16)、poke-weed cerebroside(17)、何首乌乙素(18)、9,10,13-三羟基十八烷酸(19)、正丁基-β-D-吡喃果糖苷(20)。化合物11对HCT116、SW620细胞的IC 50值分别为(30.87±2.7)、(56.11±0.49)μmol/L,化合物12对EV-A71病毒的抑制率为50.21%。结论化合物1为新天然产物,化合物9~10为首次从蓼科植物中分离得到,化合物8、12、14、17、19为首次从何首乌属植物中分离得到,化合物2、4~7、11、18为首次从该植物中分离得到。化合物11具有细胞毒性,化合物12具有抗病毒活性。AIM To study the chemical constituents from Fallopia multiflora var.ciliinervis(Nakai)Yonekura&H.Ohashi and their in vitro cytotoxic,anti-viral activities.METHODS The 75%ethanol extract from F.multiflora var.ciliinervis was isolated and purified by silica and semi-preparative HPLC,then the structures of obtained compounds were identified by physicochemical properties and spectral data.The in vitro cytotoxic activities were measured by MTT method,and the in vitro anti-viral activities were measured by CCK-8 method.RESULTS Twenty compounds were isolated and identified as 3-acetyl-4,5-dimethoxy-benzoic acid(1),methylgallate(2),gallic acid(3),p-hydroxybenzaldehyde(4),protocatechuic acid(5),3,4-dihydroxybenzaldehyde(6),syringic acid(7),4-hydroxy-3-methoxybenzoic acid(8),p-hydroxyphenethyl-O-β-D-glucoside(9),2-(4-hydroxy-3-methoxyphenyl)-ethyl-O-β-D-glucopyranoside(10),trans-resveratrol(11),cis-resveratrol(12),resveratrol-3-O-β-D-(trans-2′-O-cinnamoyl)-glucoside(13),resveratrol-3-O-β-D-(cis-2′-O-cinnamoyl)-glucoside(14),resveratrol-3-O-β-D-glucoside(15),N-trans-feruloyl-3-methyldopamine(16),poke-weed cerebroside(17),polygonimitin B(18),9,10,13-trihydroxyoctadecanoic acid(19),n-butyl-β-D-fructopyranoside(20).Compound 11 showed cytotoxic activities against HCT116 and SW620 cells with IC 50 values of(30.87±2.7)μmol/L and(56.11±0.49)μmol/L,respectively,and the inhibitory rate of compound 12 on EV-A71 virus was 50.21%.CONCLUSION Compound 1 is a new natural product,compounds 9-10 are the first isolation from family Polygonaceae,compounds 8,12,14,17,19 are the first isolation from genus Fallopia,compounds 2,4-7,11,18 are the first isolation from this plant.Compound 11 possesses cytotoxic activity,while compound 12 displays anti-viral activity.

关 键 词：朱砂七 化学成分 分离鉴定 细胞毒性 抗病毒活性 

分 类 号：R284.1[医药卫生—中药学]