高效液相色谱法同时测定化妆品中辣椒碱等9种热感剂  

Simultaneous Determination of 9 Self-heating Compounds Including Capsaicin in Cosmetics by High Performance Liquid Chromatography

作　　者：吴志珊 吴姣娇 尹伟成 殷帅 李卓芳 姜成君 WU Zhi-shan;WU Jiao-jiao;YIN Wei-cheng;YIN Shuai;LI Zhuo-fang;JIANG Cheng-jun(Hunan Institute for Drug Control,Changsha 410001,China;Food and Drug Inspection in Yueyang,Yueyang 414000,China)

机构地区：[1]湖南省药品检验检测研究院,湖南长沙410001 [2]岳阳市食品药品检验所,湖南岳阳414000

出　　处：《分析测试学报》2023年第5期641-646,共6页Journal of Instrumental Analysis

摘　　要：建立了测定化妆品中辣椒碱等9种热感剂的高效液相色谱法。样品经80%甲醇超声提取后,过滤,以乙腈-0.5%甲酸水溶液为流动相,采用SymmetryShieldTMRP18色谱柱(250 mm×4.6 mm,5μm)分离,液相色谱分离,二极管阵列检测器进行检测。在优化实验条件下,9种热感剂的分离度好,专属性高,其质量浓度在0.2~100μg/mL范围内与峰面积呈良好线性关系,相关系数(r2)均不小于0.999 5,检出限(LOD)为0.001 0~0.003 4 mg/kg。以水基类、乳剂类、膏霜类3种阴性样品作为空白基质,9种热感剂在0.004、0.02、1 mg/kg 3种加标水平下的回收率为75.6%~115%,相对标准偏差(RSD)为0.083%~7.0%。该方法专属性强、灵敏度高、准确度好,可用于化妆品中9种热感剂含量的测定。A high performance liquid chromatographic(HPLC)method was established for the determi⁃nation of 9 self-heating compounds,including capsaicin in cosmetics.The samples were ultrasonically extracted with 80%methanol,and then filtered.The target compounds were separated on a Symme⁃tryShieldTMRP18(250 mm×4.6 mm,5μm)with a mobile phase consisting of acetonitrile and 0.5%formic acid by gradient elution,and analyzed by HPLC with a PDA detector.Under the optimal condi⁃tions,good linear relationships were obtained for nine compounds in the concentration range of 0.2-100μg/mL,with correlation coefficients(r2)not less than 0.9995.The limits of detection(LODs)were in the range of 0.0010-0.0034 mg/kg.The spiked recoveries for water,lotion and cream ma⁃trixes at three levels of 0.004,0.02,1 mg/kg ranged from 75.6%to 115%,with relative standard de⁃viations(RSDs,n=3)of 0.083%-7.0%.This method is accurate and sensitive,and is suitable for the qualitative and quantitative analysis of 9 self-heating compounds in cosmetics.

关 键 词：热感剂 化妆品 高效液相色谱法(HPLC) 

分 类 号：O657.72[理学—分析化学] S641.3[理学—化学]