肾病综合征患者尿足细胞与临床病理关系的研究  

Study on Relationship Between Clinical Pathology and Urine Podocyte in Patients with Nephrotic Syndrome

作　　者：丁华君 贺文敏 鲍晓帅 应琴琴 褚宇东[2] Ding Hua-jun;He Wen-min;Bao Xiao-shuai;Ying Qin-qin;Chu Yu-dong(Second Department of Internal Medicine,Ninghai Chengguan Hospital,Ningbo 315100,Zhejiang Province,China;Department of Nephrology,Ningbo Medical Center Lihuili Hospital Eastern District,Ningbo 315200,Zhejiang Province,China)

机构地区：[1]宁海县城关医院内二科,浙江宁波315100 [2]宁波市医疗中心李惠利医院东部院区肾内科,浙江宁波315200

出　　处：《中国社区医师》2023年第10期100-102,105,共4页Chinese Community Doctors

摘　　要：目的:探讨局灶节段性肾小球硬化(FSGS)、微小病变型肾病(MCD)、膜性肾病(MN)患者尿足细胞与临床病理之间的关系。方法:选取2017年6月—2021年6月浙江省宁波市宁海县城关医院内二科及宁波市医疗中心李惠利医院东部院区肾内科80例患者作为研究对象,经肾活检病理检查确诊分为FSGS组、MCD组、MN组,另选同期30例体检健康人员作为对照组。收集四组一般临床资料、生化指标,采用免疫荧光、免疫印迹实验、核酸检测检测四组患者治疗前后尿中的足细胞表达。比较四组一般临床资料、生化指标、尿足细胞表达与阳性率。结果:四组年龄比较,差异有统计学意义(P=0.035);FSGS组、MCD组、MN组收缩压高于对照组,差异有统计学意义(P=0.032);FSGS组以急性肾损伤入院的患者比例高于MCD组、MN组,差异有统计学意义(P=0.023)。FSGS组、MCD组、MN组白蛋白、肾小球滤过率低于对照组,低密度脂蛋白、尿酸、24 h尿蛋白定量水平高于对照组,差异有统计学意义(P<0.05)。对照组未检出尿足细胞,FSGS组尿足细胞阳性率高于MCD组、MN组、对照组,差异有统计学意义(P<0.05)。结论:根据患者尿足细胞含量在一定程度上可推测肾病综合征患者的病理分型。Objective:To investigate the relationship between urine podocyte and clinical pathology in patients with focal segmental glomerulosclerosis(FSGS),minimal change nephrosis(MCD)and membranous nephropathy(MN).Methods:From June 2017 to June 2021,80 patients from the Second Department of Internal Medicine,Ninghai Chengguan Hospital in Ningbo City of Zhejiang Province and the Department of Nephrology of Ningbo Medical Center Lihuili Hospital Eastern District were selected as study subjects.Upon the confirmed diagnosis by pathological examination of renal biopsy,the patients were divided into FSGS group,MCD group and MN group.In addition,30 healthy subjects for physical examination during the same period were selected as the control group.The general clinical data and biochemical indexes in four groups were collected.The expression of podocyte in the urine before and after treatment in four groups was detected by immunofluorescence,immunoblotting assay,and nucleic acid testing.The general clinical data,biochemical indexes,urine podocyte expression and positive rate in the four groups were compared.Results:The age in the four groups showed statistically significant difference(P=0.035).The systolic blood pressure in FSGS group,MCD group and MN group was higher than that in the control group,and the difference was statistically significant(P=0.032).The proportion of patients admitted to the hospital due to acute kidney injury in the FSGS group was higher than that in the MCD group and the MN group,and the difference was statistically significant(P=0.023).The albumin and glomerular filtration rates in FSGS group,MCD group and MN group were lower than those in the control group,and the low-density lipoprotein,uric acid and 24-hour urine protein quantitative levels in FSGS group,MCD group and MN group were higher than those in the control group,and the differences were statistically significant(P<0.05).No podocyte was detected in the control group,and the positive rate of podocyte in the FSGS group was higher than that in the

关 键 词：局灶节段性肾小球硬化 微小病变型肾病 膜性肾病 尿足细胞 

分 类 号：R692[医药卫生—泌尿科学]