‘红颜’草莓离体叶片失水及抗旱生理生化特性  

Water loss and the physiological and biochemical characteristics of strawberry detached leaves potential to drought resistance

作　　者：李金羽 石硕 宋思豪 遆月迎 沈元月[1] 郭家选[2] 高凡[2] LI Jinyu;SHI Shuo;SONG Sihao;TI Yueying;SHEN Yuanyue;GUO Jiaxuan;GAO Fan(Beijing Key Laboratory of New Agricultural Application Technology/School of Plant Science and Technology of Beijing Agricultural University,Beijing 102206,China;Key Laboratory of Urban Agriculture in North China,Ministry of Agriculture and Rural Affairs/Department of Resources and Environment,Beijing University of Agriculture,Beijing 102206,China)

机构地区：[1]农业应用新技术北京市重点实验室/北京农学院植物科学技术学院,北京102206 [2]农业农村部华北都市农业重点实验室/北京农学院资源与环境系,北京102206

出　　处：《北京农学院学报》2023年第2期44-48,共5页Journal of Beijing University of Agriculture

基　　金：国家自然科学基金资助项目(32072516;32272648);北京市自然科学基金-市教委联合资助项目(KZ202010020028)。

摘　　要：【目的】探究草莓离体叶片的失水特性及相关生理生化指标,为草莓耐旱性提供依据。【方法】采取离体叶片自然失水法,测定草莓叶片的净光合速率(P_(n))、蒸腾速率(T_(r))、气孔导度(G_(s))、胞间CO_(2)浓度(C i)、叶绿素荧光参数、过氧化氢(H_(2)O_(2))、丙二醛(MDA)含量和相对电导率(EC)、过氧化氢酶(CAT)、过氧化物酶(POD)及超氧化物歧化酶(SOD)等指标。【结果】在脱水条件下4 h时间范围内,草莓离体叶片与非离体叶片(CK)相比,相对含水量、P_(n)、T_(r)、G_(s)、最大荧光(F_(m))、最大光化学效率(F_(v)/F_(m))快速下降,而叶绿素荧光参数的初始荧光(F_(0))显著上升;草莓离体叶片H_(2)O_(2)与MDA含量显著上升,叶片EC增大,并在4 h时达到顶峰,与对照相比,分别上升84.9%、77.1%、103.1%;同时POD、SOD与CAT活性显著升高。【结论】快速脱水对草莓叶片的光合器官、细胞膜系统造成严重损害,通过增强离体叶片的抗氧化酶活性,维持叶片细胞膜的相对稳定。[Objective]The characteristics of water loss and relative physiological and biochemical parameters in detached strawberry leaves were investigated to provide the basis for evaluation of drought tolerance.[Methods]The net photosynthetic rate(P_(n)),transpiration rate(T_(r)),stomatal conductance(G_(s)),intercellular CO_(2)concentration(C i),chlorophyll fluorescence parameters,hydrogen peroxide(H_(2)O_(2)),malondialdehyde(MDA)content and relative electrical conductivity(EC),Catalase(CAT),peroxidase(POD)and superoxide dismutase(SOD),and other indicators.[Results]In the time range of 4 h under dehydration,RWC,P_(n),T_(r),G_(s),maximum fluorescence(F_(m))and maximum photochemical efficiency(F_(v)/F_(m))of isolated strawberry leaves decreased rapidly compared with non-isolated leaves(CK),while the initial fluorescence(F_(0))of chlorophyll fluorescence parameters increased significantly;H_(2)O_(2)and MDA contents of isolated strawberry leaves increased significantly,and leaf EC increased and peaked at The H_(2)O_(2)and MDA contents of strawberry isolated leaves increased significantly,and the leaf EC increased and peaked at 4 h,rising 84.9%,77.1%and 103.1%,respectively,compared with the control;meanwhile,the POD,SOD and CAT activities increased significantly.[Conclusion]Rapid dehydration caused serious damage to photosynthetic organs and cell membrane system of strawberry leaves,and the relative stability of leaf cell membrane was maintained by enhancing the antioxidant enzyme activities of isolated leaves.

关 键 词：草莓 离体叶片 保水力 生理生化 

分 类 号：S668.4[农业科学—果树学]