儿童传染性单核细胞增多症EB病毒载量及外周血T淋巴细胞亚群分布与肝损伤的相关性  

Correlation Between Epstein-Barr Viral Load and Peripheral Blood T Lymphocyte Subsets and Liver Injury in Children with Infectious Mononucleosis

作　　者：王志银[1] 王世岩 刘京硕 宋琛 张海光 WANG Zhiyin;WANG Shiyan;LIU Jingshuo;SONG Chen;ZHANG Haiguang(Department of Laboratory Medicine,the First Affiliated Hospital of Xinxiang Medical University,Weihui 453100,China;Department of Gynaecology and Obstetrics,the First Affiliated Hospital of Xinxiang Medical University,Weihui 453100,China)

机构地区：[1]新乡医学院第一附属医院检验科,河南卫辉453100 [2]新乡医学院第一附属医院妇产科,河南卫辉453100

出　　处：《河南医学研究》2023年第9期1608-1611,共4页Henan Medical Research

基　　金：河南省医学科技攻关计划联合共建项目(LHGJ20200498)。

摘　　要：目的 探讨传染性单核细胞增多症(IM)患儿EB病毒(EBV)载量及外周血T淋巴细胞亚群分布与肝功能损伤的相关性。方法 选取2021年1月至2022年12月新乡医学院第一附属医院收治的IM患儿40例为IM组,并选取同期40例健康体检儿为对照组。检测并比较两组儿童EBV DNA载量、CD3^(+)、CD3^(+)CD4^(+)、CD3^(+)CD8^(+)、CD4^(+)/CD8^(+)、白细胞计数(WBC)、中性粒细胞百分比(Net%)、淋巴细胞百分比(LYM%)、单核细胞百分比(Mon%)及肝功能指标谷丙转氨酶(ALT)及谷草转氨酶(AST)。分析IM组患儿EBV DNA载量、T淋巴细胞亚群分布与ALT、AST的相关性。结果 IM组WBC、LYM%高于对照组,Net%低于对照组(P<0.05);两组Mon%差异无统计学意义(P>0.05)。IM组CD3^(+)、CD3^(+) CD8^(+)比率均高于对照组,CD3^(+)CD4^(+)比率及CD4^(+)/CD8^(+)均低于对照组(P<0.05)。IM组EBV DNA载量、ALT、AST水平均高于对照组(P<0.05)。IM患儿的EBV DNA载量与ALT和AST水平均呈正相关(r=0.345,P=0.023;r=0.382,P=0.010),CD3^(+)比率与ALT及AST水平均呈正相关(r=0.409,P=0.010;r=0.353,P=0.030),CD3^(+)CD4^(+)比率与AST水平呈正相关(r=0.334,P=0.038)。结论 IM患儿EBV DNA载量升高、CD3^(+)T细胞比率上升、CD3^(+)CD4^(+)T细胞比率降低均与患儿并发肝损伤有一定相关性。Objective To investigate the correlation between Epstein-Barr viral(EBV)load,peripheral blood T lymphocyte subsets distribution and liver function injury in children with infectious mononucleosis(IM).Methods Forty children with IM who admitted to the First Affiliated Hospital of Xinxiang Medical College from January 2021 to December 2022 were selected as the IM group,and 40 healthy children during the same period were selected as the control group.EBV DNA load,CD3^(+),CD3^(+)CD4^(+),CD3^(+)CD8^(+),CD4^(+)/CD8^(+),white blood cell count(WBC),neutrophil percentage(Net%),lymphocyte percentage(LYM%),monocyte percentage(Mon%)and liver function indexes alanine transaminase(ALT)and aspartate aminotransferase(AST)were detected and compared between the two groups.The correlation between EBV DNA load,T lymphocyte subsets distribution and ALT and AST in IM group was analyzed.Results WBC and LYM%in IM group were higher than those in control group,and Net%was lower than those in control group,the differences were statistically significant(P<0.05),and there was no statistically significant difference in Mon%(P>0.05).The percentages of CD3^(+)and CD3^(+)CD8^(+)in IM group were higher than those in control group,and the percentages of CD3^(+)CD4^(+)and CD4^(+)/CD8^(+)were lower than those in control group(P<0.05).The EBV DNA load,ALT and AST levels in IM group were higher than those in control group(P<0.05).There was a positive correlation between EBV DNA load and ALT and AST levels in IM children(r=0.345,P=0.023;r=0.382,P=0.010),the CD3^(+)ratio was positively correlated with ALT and AST levels(r=0.409,P=0.010;r=0.353,P=0.030),CD3^(+)CD4^(+)ratio was positively correlated with AST level(r=0.334,P=0.038).Conclusion The increase of EBV DNA load and CD3^(+)T cell ratio,decreased of CD3^(+)CD4^(+)T cell ratio in children with IM are associated with liver injury.

关 键 词：传染性单核细胞增多症 EB病毒 T淋巴细胞亚群 肝损伤 相关性 

分 类 号：R446.1[医药卫生—诊断学]