基于NR2B蛋白表达探究异氟醚对妊娠大鼠疼痛水平及幼鼠神经细胞活性的作用机制  

To explore the mechanism of isoflurane on pain level of pregnant rats and neural cell activity of young rats based on NR2B protein expression

作　　者：谢丽 唐国强[1] 刘迁 许滔 赵健衡 XIE Li;TANG Guoqiang;LIU Qian;XU Tao;ZHAO Jianheng(Zigong First People’s Hospital,Zigong,Sichuan 643000,China;Panzhihua Central Hospital,Panzhihua,Sichuan 617067,China)

机构地区：[1]自贡市第一人民医院,四川自贡643000 [2]攀枝花中心医院,四川攀枝花617067

出　　处：《中国优生与遗传杂志》2023年第4期715-720,共6页Chinese Journal of Birth Health & Heredity

基　　金：四川省医学(青年创新)科研项目(S19008)。

摘　　要：目的 基于NR2B蛋白表达探究异氟醚对妊娠大鼠疼痛水平及幼鼠神经细胞活性的作用机制。方法 选取40只SPF级SD孕15天雌性大鼠,随机分为正常(N)组,低异氟醚(LI)组,高异氟醚(HI)组,七氟醚(S)组,每组10只,对LI、HI组分别给予不同体积分数异氟醚并空氧混合气体吸入,对S组给予七氟醚并空氧混合气体吸入,N组同期给予同体积空氧混合气体吸入,测痛仪检测大鼠疼痛阈值,水迷宫法检测幼鼠认识功能,高尔基体染色法检测幼鼠神经细胞突触棘数量,TUNEL法检测幼鼠神经细胞凋亡,免疫组化法检测NR2B蛋白表达;另取培养后的神经细胞进行体外实验,将其分为两组,一组加入0.3 mmol/L的乳化异氟醚1 mL(观察组);另一组加入1 mL 0.3 mmol/L的NR2B激动剂(对照组)。结果 与N组比较,LI组、HI组、S组0、6、12、18、24 h的体表疼痛阈值明显升高(P<0.05),幼鼠逃逸潜伏期、神经细胞凋亡、NR2B蛋白表达明显升高(P<0.05),穿越平台次数及平台象限停留时间、神经细胞突触棘数量明显降低(P<0.05),且HI组比LI组变化明显(P<0.05),而S组与HI组相比无明显差异(P>0.05);观察组与对照组相比,神经细胞中NR2B蛋白表达无明显差异(P>0.05)。结论 异氟醚可有效减轻妊娠大鼠体表疼痛,但对幼鼠认知功能及神经细胞均有一定影响,其作用机制可能与提高NR2B水平有关。Objective To explore the mechanism of isoflurane on pain level of pregnant rats and neural cell activity of young rats based on NR2B protein expression.Methods Selected 40 SPF SD female rats at 15 days,were randomly divided into normal group(N),low isoflurane group(LI),high isoflurane group(HI),sevoflurane(S)group,each group of 10,only to LI,HI groups respectively to different volume fraction isoflurane and empty oxygen mixed gas suction,the group S giving oxygen mixed gas inhalation sevoflurane and empty.The pain threshold of rats was detected by pain meter,the cognitive function was detected by water maze method,the number of synaptic spines of nerve cells was detected by Golgi staining method,the apoptosis of nerve cells was detected by TUNEL method,and the expression of NR2B protein was detected by immunohistochemistry.In addition,cultured nerve cells were taken for in vitro experiment and divided into two groups.One group was added with 1 mL emulsified isoflurane(0.3 mmol/L)(observation group).In the other group,1 mL of 0.3 mmol/L NR2B agonist was added(control group).Results Compared with group N,the pain threshold of body surface at 0,6,12,18 and 24 h in LI,HI and S groups were significantly increased(P<0.05),and the escape latency,neuronal cell apoptosis and NR2B protein profile of young rats were significantly increased(P<0.05).The times of crossing the platform,the residence time of the platform quadrant,and the number of synaptic spines of nerve cells were significantly decreased(P<0.05),and the changes were significantly higher in HI group than in LI group(P<0.05),but there was no significant difference between S group and HI group(P>0.05).There was no significant difference in NR2B protein expression between the observation group and the control group(P>0.05).Conclusion Isoflurane can effectively relieve the pain on the body surface of pregnant rats,but it has a certain effect on the activity of nerve cells in young rats,which may be related to the activation of NR2B.

关 键 词：异氟醚 妊娠 疼痛 神经细胞 活性 

分 类 号：R614[医药卫生—麻醉学]