优化测定表观消化率指示剂二氧化钛含量前处理方法的研究  

Optimization of Pretreatment Method for Determining the Digestibility Indicator-Titanium Dioxide

作　　者：张丽萍[1] 王久荣[1] 唐思宇 袁红朝[1] 贺珍 耿梅梅[1] 许丽卫[1] 陈闻 冯淦熠 ZHANG Liping;WANG Jiurong;TANG Siyu;YUAN Hongzhao;HE Zhen;GENG Meimei;XU Liwei;CHEN Wen;FENG Ganyi(Key Laboratory of Agro-ecological Processes in Subtropical Region,Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha 410125)

机构地区：[1]中国科学院亚热带农业生态研究所/亚热带农业生态过程重点实验室,长沙410125

出　　处：《中国农学通报》2023年第11期159-164,共6页Chinese Agricultural Science Bulletin

基　　金：中国科学院任务院其他任务“技术支撑人才”(E2290801)。

摘　　要：旨在解决测定指示剂二氧化钛前处理方法描述不详细、测定结果变异系数大的问题,实现快速准确测定饲料中二氧化钛含量,以准确测定畜禽消化试验消化率,进而准确评定饲料品质。试验以秸秆饲料为研究对象,电感耦合等离子体发射光谱仪为检测仪器,优化了电热板硫酸消解法中硫酸铵用量与升温程序等条件,建立了硫酸消解-电感耦合等离子体发射光谱仪检测饲料中二氧化钛含量的方法;并比较了电热板硫酸消解、氢氟酸消解及干灰化3种前处理方法。结果表明,电热板硫酸消解法检测结果的精密度、回收率均优于氢氟酸消解法和灰化法,且钛在0~200 mg/L检测范围内,线性关系良好,相关系数为1.00;变异系数不大于1.32%;二氧化钛回收率大于97.1%。该方法简便、快捷,具有较宽的线性范围、较高的准确度和精密度,适用于消化试验中指示剂二氧化钛含量的批量测定,可为快速准确地测定养分表观消化率及评定饲料品质提供技术支撑。The study aims to solve the problems of inaccurate description of the pretreatment method for the determination of indicator titanium dioxide,which can cause large coefficient of variation of the determination results,and to realize rapid and accurate determination of titanium dioxide content,and accurately determine the digestibility of livestock and poultry digestion test and evaluate the quality of feed.Taking straw feed as the research material,and the inductively coupled plasma emission spectrometer as the detection instrument,The experiment optimized the conditions such as the ammonium sulfate dosage and heating program in the sulfuric acid digestion method,and then established a method for determining the content of titanium dioxide in feed by sulfuric acid digestion and inductively coupled plasma emission spectrometer.Three pretreatment methods were compared,which were sulfuric acid digestion,hydrofluoric acid digestion and dry ashing.The results showed that the precision and recovery rate of the test results of the sulfuric acid digestion method were better than those of the hydrofluoric acid digestion method and the dry ashing method,the linear relationship between 0-200 mg/L of titanium was good,and the correlation coefficient was 1.0;the coefficient of variation was less than 1.32%;the recovery rate of titanium dioxide was more than 97.1%.This method is simple and rapid,which has a wide linear range,high accuracy and precision.It is suitable for batch determination of indicator titanium dioxide content in digestion test,and can provide technical support for fast and accurate determination of apparent digestibility of nutrients and evaluation of feed quality.

关 键 词：饲料 二氧化钛 电感耦合等离子体发射光谱仪 消化率 指示剂 

分 类 号：O652.1[理学—分析化学]