2021-2022年苏州市乙型流感病毒的基因特征分析  

Genome characteristics of influenza B virus in Suzhou city,2021-2022

作　　者：董泽丰 杨瑞敏 刘洋[1] 王笛 徐智慧 袁轩 沈强[1] 庞媛媛[1] 夏瑜[1,2] Dong Zefeng;Yang Ruimin;Liu Yang;Wang Di;Xu Zhihui;Yuan Xuan;Shen Qiang;Pang Yuanyuan;Xia Yu(Suzhou Center for Disease Control and Prevention,Suzhou 215004,China;Nanjing Medical University,Nanjing 210000,China)

机构地区：[1]苏州市疾病预防控制中心,苏州215004 [2]南京医科大学,南京210000

出　　处：《中华实验和临床病毒学杂志》2023年第2期178-183,共6页Chinese Journal of Experimental and Clinical Virology

基　　金：2020年苏州市重大疾病预防和控制关键技术(GWZX202002);2020年苏州市姑苏卫生人才计划培养项目(GSWS202099);苏州市医学重点学科(SZXK202117);苏州市卫生青年骨干人才"全国导师制"培训项目(QNGG2022030)。

摘　　要：目的了解苏州市2021年7月至2022年1月流感流行期间乙型流感病毒(influenza B virus,FluB)的基因组和遗传进化特征。方法采用实时荧光-逆转录聚合酶链反应(Real-time fluorescence reverse transcription polymerase chain reaction,Real-time RT-PCR)法对标本进行流感病毒(influenza virus,Flu)分型检测。对检出的FluB毒株,经全基因组捕获和文库构建,利用Miseq高通量测序平台对样本进行测序。采用MEGA X软件以邻接法(Neighbor-Joining method,NJ)构建FluB血凝素(hemagglutinin,HA)、神经氨酸酶(neuraminidase,NA)和基质蛋白(matrix protein,MP)基因系统发育树;采用NetNGlyc 1.0 server软件预测HA和NA蛋白潜在N-糖基化位点。结果在送检的1500份咽拭子样本中,280份检出FluB,对其中53株样本完成了FluB全基因组序列测定。序列分析显示,FluB毒株8个基因节段(PB1、PB2、PA、HA、NP、NA、MP、NS)核苷酸序列相似性分别为99.3%~100%、98.1%~100%、98.8%~100%、98.0%~100%、99.2%~100%、98.4%~100%、98.2%~100%、99.0%~100%。除2021年7月4份样本的FluB为V1A.3进化分支外,其余样本均为V1A.3a.2进化分支。与疫苗株B/Washington/02/2019相比,2021年10月份以后样本的FluB HA蛋白氨基酸序列存在9~11个差异位点,共享9个突变位点(H122Q、A127T、R133G、P144L、N150K、G184E、N197D、K203R、R279K);未发现奥司他韦等NA抑制剂相关耐药突变。FluB HA和NA蛋白分别具有11个和4个潜在糖基化位点。结论2021年7月至2022年1月期间,苏州市流行的FluB以V1A.3a.2毒株为优势流行株,HA和NA蛋白氨基酸序列发生多位点突变。Objective To understand the genome and genetic evolution characteristics of influenza B virus(FluB)in Suzhou city from July 2021 to January 2022.Methods Real-time fluorescence reverse transcription polymerase chain reaction(Real-Time RT-PCR)was used for the typing of influenza virus(Flu).The detected FluB strains were sequenced by Miseq high-throughput sequencing platform through whole genome capture and library construction.The FluB hemagglutinin(HA),neuraminidase(NA)and matrix protein(MP)gene phylogenetic trees were constructed by Neighbor-Joining method(NJ)with MEGA X software.The Potential N-glycosylation sites of HA and NA proteins were predicted by NetNGlyc 1.0 server software.Results FluB was detected in 280 of the 1500 throat swab samples,and the FluB genome sequence was completed in 53 strains.The nucleic acid identity of 8 gene fragments(PB1,PB2,PA,HA,NP,NA,MP,NS)in the FluB strains was 99.3%-100%,98.1%-100%,98.8%-100%,98.0%-100%,99.2%-100%,98.4%-100%,98.2%-100%and 99.0%-100%,respectively.Except for the 4 samples in July 2021,which belonged to the V1A.3 clade of FluB,the rest of the samples belonged to the V1A.3a.2 clade.Every amino acid sequence of HA protein of Flu B collected after October 2021 showed 9-11 substitutions compared with the reference strain(B/Washington/02/2019),which sharing 9 mutation sites(H122Q,A127T,R133G,P144L,N150K,G184E,N197D,K203R and R279K).No drug-resistant mutations associated with NA inhibitors such as oseltamivir were found.Respectively,11 and 4 potential glycosylation sites were identified in HA and NA proteins of the FluB strains.Conclusions From July 2021 to January 2022,V1A.3a2 was the dominant FluB strains in Suzhou city,and the amino acid sequences of HA and NA proteins showed multiple site mutations.

关 键 词：乙型流感病毒 血凝素 神经氨酸酶 突变 糖基化 

分 类 号：R373.13[医药卫生—病原生物学]