2021年湖南省人民医院新生儿副肠孤病毒的遗传进化分析  

Analysis of genetic evolution of parechovirus in neonates from Hunan Provincial People’s Hospital in 2021

作　　者：马晓华 谢乐云[3] 柴萨萨 高升辉 张爱民[3] 余阗[3] 郑贵森 李利利[2] 曾赛珍[3] 段招军[2] Ma Xiaohua;Xie Leyun;Chai Sasa;Gao Shenghui;Zhang Aimin;Yu Tian;Zheng Guisen;Li Lili;Zeng Saizhen;Duan Zhaojun(School of Public Health,Gansu University of Chinese Medicine,Lanzhou 730000,China;National Institute for Viral Disease Control and Prevention,China CDC,Beijing 100052,China;Hunan Provincial People's Hospital,The First Affiliated Hospital of Hunan Normal University,Changsha 410005,China;School of Basic Medicine,North China University of Science and Technology,Tangshan 063000,China)

机构地区：[1]甘肃中医药大学公共卫生学院,兰州730000 [2]中国疾病预防控制中心病毒病预防控制所,北京100052 [3]湖南省人民医院湖南师范大学附属第一医院,长沙410005 [4]华北理工大学基础医学院,唐山063000

出　　处：《中华实验和临床病毒学杂志》2023年第2期184-188,共5页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的初步了解湖南省人民医院新生儿中副肠孤病毒的流行情况,分析其遗传进化特征。方法2021年6~9月份在湖南省人民医院采集住院新生儿粪便标本,采用TaqMan real-time qPCR和RT-PCR方法进行人副肠孤病毒(human parechovirus,HPeV)的筛查和分型,采用高通量测序和RT-PCR方法扩增其全基因组,序列测定后进行系统发育分析。结果共采集新生儿粪便样本123份,其中HPeV阳性为22份,阳性率为17.89%,分型结果显示所有毒株均属于HPeV-1基因型;扩增获得1株HPeV基因组全长为7269 bp,命名为Hunan/HPeV/2021,该基因组具有典型的HPeV基因组结构;序列比对结果显示该毒株与GenBank中已知的HPeV-1型的基因组核苷酸序列具有最高同源性,为86.6%~91.9%;其编码的唯一的长开放阅读框(open reading frame,ORF)长度为6540 nt,与已知相似序列的核苷酸和氨基酸同源性分别为90.3%~92.6%和97.3%~98.3%;系统发育分析显示该病毒株属于HPeV-1型进化分支,与我国流行的HPeV-1型毒株聚为同一分支。结论在湖南省人民医院新生儿中HPeV具有较高的检出率且均为HPeV-1型。Objective To understand the prevalence of human parechovirus(HPeV)in neonates of Hunan Provincial People’s Hospital,and analyze genetic evolutionary characteristics.Methods From June to September 2021,fecal samples of inpatient neonates were collected in Hunan Provincial People′s Hospital.TaqMan real-time qPCR and RT-PCR were used for HPeV screening and genotyping.High-throughput sequencing and PCR were used to obtain whole genomes.Phylogenetic analysis was performed after sequencing.Results A total of 123 fecal samples of neonates were collected,of which 22 were HPeV positive,with 17.89%positive rate.All the strains belonged to the HPeV-1 genotype.One full-length genomic sequence of 7269 bp were obtained,and provisionally named Hunan/HPeV/2021,which has the highest nucleotide identity with known HPeV-1 genotype,with 86.6%-91.9%nucleotide identity.The nucleotide and amino acid identity of open reading frame(ORF)with known similar sequences were 90.3%-92.6%and 97.3%-98.3%,respectively.The phylogenetic analysis showed that Hunan/HPeV/2021 belongs to the HPeV-1 genotype,which is clustered into the same clade as the popular HPeV-1 strains in China.Conclusions HPeV has a high prevalence in inpatient neonates of Hunan Provincial People’s Hospital and belong to the HPeV-1 genotype.

关 键 词：新生儿 副肠孤病毒 遗传进化分析 

分 类 号：R722.1[医药卫生—儿科]