陆地棉膜结合脂肪酸去饱和酶基因家族的全基因组鉴定及表达分析  

Genome-Wide Identification and Expression Analysis of Membrane-Bound Fatty Acid Desaturase Gene Family in Upland Cotton(Gossypium hirsutum L.)

作　　者：陈义珍[1] 李浩[1] 傅明川 王立国 刘任重[1] 柳展基[1] Chen Yizhen;Li Hao;Fu Mingchuan;Wang Liguo;Liu Renzhong;Liu Zhanji(Key Laboratory of Cotton Breeding and Cultivation in Huang-Huai-Hai Plain,Ministry of Agriculture and Rural Affairs/Institute of Industrial crops,Shandong Academy of Agricultural Sciences,Jinan 250100,China)

机构地区：[1]农业部黄淮海棉花遗传改良与栽培生理重点实验室/山东省农业科学院经济作物研究所,山东济南250100

出　　处：《山东农业科学》2023年第4期11-23,共13页Shandong Agricultural Sciences

基　　金：山东省农业良种工程项目(2020LZGC002);国家自然科学基金项目(31901422);山东省农业科学院农业科技创新工程项目(CXGC2021A46)。

摘　　要：膜结合脂肪酸去饱和酶(fatty acid desaturase,FAD)是生物合成不饱和脂肪酸的关键酶。本研究以陆地棉基因组数据为基础,利用生物信息学方法对棉花FAD基因家族进行全基因组鉴定和进化分析。结果表明,陆地棉基因组中共含有37个FAD基因,进化分析发现这些基因分为4个亚家族,各亚家族成员数量不一,但相同亚家族成员含有相似的基因结构和保守基序。共线性分析发现28对复制基因全为片段复制基因,且都经历了严格的纯化选择作用。此外,在陆地棉FAD基因的启动子区域,发现了丰富的响应植物激素信号和逆境胁迫的顺式作用元件。转录组分析表明,陆地棉FAD基因家族响应干旱和盐胁迫,定量PCR分析表明施加外源褪黑素显著影响了FAD基因的表达。本研究结果为进一步解析FAD家族基因的功能奠定了基础。Membrane-bound fatty acid desaturases(FADs)are the key enzymes for the biosynthesis of unsaturated fatty acids.In this study,the whole genome of FAD gene family was identified and its evolution was analyzed by bioinformatics method based on the genomic data of upland cotton(Gossypium hirsutum L.).The results showed that there were 37 FAD genes in the upland cotton genome.Phylogenetic analyses indicated these FAD genes were divided into four subfamilies,and each contained different numbers of FAD genes,but the members in the same subfamily contained similar gene structure and conserved motifs.Collinear analysis revealed that all the 28 pairs of replicators were fragment replicators and had undergone rigorous purification and selection.In addition,abundant cis-acting elements related to phytohormone signaling and stress responses were identified in the promoter regions of FAD genes.Transcriptome analysis showed that the FAD gene family of upland cotton responded to drought and salt stress,and quantitative PCR analysis showed that exogenous melatonin significantly affected the FAD gene expression.The results of this study laid a foundation for further functional analysis of FAD family genes.

关 键 词：陆地棉 膜结合脂肪酸去饱和酶 全基因组鉴定 生物信息学分析 基因表达 干旱和盐胁迫 褪黑素 

分 类 号：S562[农业科学—作物学] Q78[生物学—分子生物学]