模拟增温对青海湖高寒湿地产甲烷菌群落特征的影响  

The influence of simulated warming on the community characteristics of methanogenic bacteria in Qinghai Lake alpine wetland

作　　者：章妮 陈克龙 祁闻[2,3] 崔博亮 ZHANG Ni;CHEN Kelong;QI Wen;CUI Boliang(College of Life Sciences Qinghai Normal University,Xining 810008,China;Qinghai Key Laboratory of Physical Geography and Environmental Processes,Xining 810008,China;Key Laboratory of Tibetan Plateau Land Surface Processes and Ecological Conservation(Ministry of Education),Xining 810008,China)

机构地区：[1]青海师范大学生命科学学院,西宁810008 [2]青海省自然地理与环境过程重点实验室,西宁810008 [3]青藏高原地表过程与生态保育教育部重点实验室,西宁810008

出　　处：《生态科学》2023年第4期163-170,共8页Ecological Science

基　　金：青海省创新平台建设专项青海省自然地理与环境过程重点实验室(2020-ZJ-Y06);第二次青藏高原综合科学考察研究(2019QZKK0405);国家自然科学基金项目(41661023);国家科技支撑计划项目(2017YFC0403601)。

摘　　要：高寒湿地生态系统作为重要的甲烷排放源,对全球气候变暖有着较大的贡献率。湿地的产甲烷菌群落在温室气体排放中扮演着重要角色,探究高寒湿地产甲烷菌群落结构及多样性对温度升高的响应十分重要。以青海湖高寒湿地为研究对象,采用高通量测序方法开展研究。基于97%的序列相似度聚类,10个土壤样品的总OTU数量为602个,对照与增温处理样品的特有OTU数分别为30和43。与对照相比,增温处理并未显著改变高寒湿地产甲烷菌群落的多样性指数及目水平优势菌群(P>0.05)。高寒湿地产甲烷菌均为广古菌门,目水平以甲烷微菌目(Methanomicrobiales,67.35%)、八叠球菌目(Methanosarcinales,14.14%)、甲烷杆菌目(Methanobacteriales,10.21%)为优势菌群。Lefse分析表明,增温后Methanomassiliicoccus相对丰度显著降低、Candidatus Methanogranum相对丰度显著升高。整体而言,青海湖高寒湿地产甲烷菌群落组成及多样性较为稳定,温度升高对产甲烷菌群落影响较小,Methanomassiliicoccus和Candidatus Methanogranum对温度变化较为敏感。As an important methane emission source,alpine wetland ecosystem contributes a great deal to global warming.Methanogen communities in wetlands play an important role in greenhouse gas emissions,and it is important to explore the response of methanogen community structure and diversity to temperature rise in alpine wetlands.This paper takes Qinghai Lake alpine wetland as the research object and adopts high-throughput sequencing method to carry out the research.Based on 97%sequence similarity clustering,the total number of OTU in the 10 soil samples is 602,and the unique number of OTU in the control and warming samples is 30 and 43,respectively.Compared with the control group,the warming treatment can not significantly change the diversity index and order level dominant flora of methanogens in the alpine wetland(P>0.05).The methanogens in the alpine wetland are all Euryarchaeota,and the order level is dominated by Methanomicrobiales(67.35%),Methanosarcinales(14.14%)and Methanobacteriales(10.21%).Lefse analysis showed that,after increasing the temperature,the relative abundance of Methanomassiliicoccus decreases significantly,while that of Candidatus Methanogranum increases significantly.On the whole,the composition and diversity of methanogen community in Qinghai Lake alpine wetland are relatively stable,and temperature increase has little effect on methanogen community,Methanomassiliicoccus and Candidatus Methanogranum are more sensitive to temperature change.

关 键 词：模拟增温 青海湖 高寒湿地 产甲烷菌 MCRA 

分 类 号：Q938.11[生物学—微生物学]