深海真菌Aspergillus puniceus A2次级代谢产物及其药物开发潜力  

Secondary metabolites of a deep-sea-derived fungus Aspergillus puniceus A2 and their potential in drug development

作　　者：蔡松岩 何建林 牛四文 洪碧红 CAI Songyan;HE Jianlin;NIU Siwen;HONG Bihong(College of Food Sciences&Technology,Shanghai Ocean University,Shanghai 201306,China;Technology Innovation Center for Exploitation of Marine Biological Resources,Third Institute of Oceanography,MNR,Xiamen 361005,China)

机构地区：[1]上海海洋大学食品学院,上海201306 [2]自然资源部第三海洋研究所海洋生物资源开发利用工程技术创新中心,福建厦门361005

出　　处：《应用海洋学学报》2023年第2期203-209,共7页Journal of Applied Oceanography

基　　金：福建省科技计划农业引导性(重点)项目(2020N0030);国家自然科学基金(82073763)。

摘　　要：本研究采用硅胶柱层析、高效液相色谱等方法对深海真菌Aspergillus puniceus A2发酵产物进行了化学成分分离纯化,通过核磁共振等现代波谱学方法,同时结合相关文献进行数据分析,鉴定化合物的结构。从A.puniceus A2中分离鉴定出6个呫吨酮并双呋喃环类化合物,化学结构分别确定为Austocystin I(1)、Austocystin G(2)、6-methoxyl Austocystin A(3)、Austocystin F(4)、F02ZA-1593B2(5)、Austocystin A(6),化合物1~6均为首次从该真菌中分离得到。通过细胞毒性和抗炎活性评价发现化合物1、2、4和6在20μmol/L浓度时,对肝星状细胞LX2显示出毒性作用,提示其可能有抗肝纤维化的作用;化合物2、3、5和6在20μmol/L浓度时,能显著抑制LPS诱导小鼠单核巨噬细胞RAW264.7细胞分泌NO,显示出一定的抗炎活性。The fermentation product of a deep-sea-derived fungus Aspergillus puniceus A2 was isolated and purified with silica gel column and high-performance liquid chromatography,which isolated 6 compounds.The chemical structures of the 6 compounds were identified as Austocystin I(1),Austocystin G(2),6-methoxyl Austocystin A(3),Austocystin F(4),F02ZA-1593B 2(5),and Austocystin A(6)by NMR spectrum analysis and compared with literature data.All the isolated compounds were reported firstly in this fungus.Among them,compounds 1,2,4 and 6 showed toxic effects on hepatic stellate cells LX2 at the concentration of 20μmol/L,suggesting that they may have anti-hepatic fibrosis effects.Compounds 2,3,5 and 6 inhibited significantly the LPS-induced NO secretion in mouse monocyte macrophage RAW264.7 at 20μmol/L,showing anti-inflammatory activity.

关 键 词：海洋生物学 深海真菌 Aspergillus puniceus 呫吨酮并双呋喃环类化合物 细胞毒活性 抗炎活性 

分 类 号：P735[天文地球—海洋生物学]