CO_(2)加富条件下胡萝卜光合通路及差异基因表达分析  

Photosynthetic Pathway and Differential Gene Expression Analysis of Carrot under CO_(2) Enrichment

作　　者：宋甜月 刘伟 宋红霞[1] SONG Tianyue;LIU Wei;SONG Hongxia(College of Horticulture,Shanxi Agricultural University,Taigu 03801,China)

机构地区：[1]山西农业大学园艺学院,山西太谷030801

出　　处：《山西农业科学》2023年第5期478-484,共7页Journal of Shanxi Agricultural Sciences

基　　金：山西省现代农业产业技术体系建设专项;山西农业大学科技创新基金项目(2016ZZ13,2005042)。

摘　　要：为了探究CO_(2)加富条件下胡萝卜光合通路及相关差异基因表达,试验在日光温室中分别设置富碳区(FC,CO_(2)浓度为(800±50)μmol/mol)和对照区(CK,自然环境),于收获期测定产量,处理61 d后收集胡萝卜叶片进行转录组测序,并对富碳处理后的差异表达基因进行KEGG注释和代谢途径分析,最后对gene33346、gene33340和gene33386等3个光合相关基因进行实时荧光定量PCR验证。结果表明,CO_(2)加富条件下总生物学产量、地上部鲜质量和地下部鲜质量均显著高于对照区,且较对照区增产36.56%。转录组结果表明,各样品的Q30碱基百分比均不小于95.24%,说明测量结果较为准确,可用于下一步数据分析;差异表达基因KEGG富集结果表明,光合通路为显著富集的第一条通路,说明CO_(2)加富对光合产生了显著影响。光合相关基因在富碳条件下上调表达,推测可能是由于其参与了光合过程中的电子传递和ATP的合成;其中gene33346、gene33340和gene33386等3个光合相关基因的相对表达量与其FPKM值趋势一致。综上,合理增施CO_(2)可以通过上调表达光合相关基因、促进光合作用,进一步提高胡萝卜产量。To investigate carrot photosynthetic pathway and related differential gene expression under CO_(2) enrichment condition,in this study,a carbon-rich area(FC)with a CO_(2) concentration of(800±50)μmol/mol and a control area(CK)in natural environment were established in a solar greenhouse.Yield was measured at the harvest period,carrot leaves were collected after 61 d for transcriptome sequencing.Differentially expressed genes after carbon enrichment were annotated using KEGG annotation and subjected to metabolic pathway analysis.Additionally,RT-qPCR was used to verify the expression of three photosynthesis-related genes,gene33346,gene33340 and gene33386.The results showed that the total biological yield,fresh weight of aboveground parts and fresh weight of underground parts were significantly higher in the FC area than those in CK area.The yield increased by 36.56%compared with the control area.The transcriptome data analysis found that the percentage of Q30 bases in each sample was not less than 95.24%,indicating measurement results were reliable and could be used for further data analysis.The enrichment results of differentially expressed gene KEGG indicated that the photosynthetic pathway was the first pathway of significant enrichment,suggesting that carbon dioxide enrichment had a significant impact on photosynthesis.Photosynthesis-related genes were up-regulated under carbon-rich conditions,it was inferred that it could be participating in electron transfer and ATP synthesis during photosynthesis.The relative expression levels of three photosynthesisrelated genes,gene33346,gene33340 and gene33386,were consistent with their FPKM values.In conclusion,reasonable increase of CO_(2) could promote photosynthesis and further improve carrot yield by up-regulating the expression of photosynthesisrelated genes.

关 键 词：胡萝卜 富碳 光合 转录组分析 

分 类 号：S631.2[农业科学—蔬菜学]