胶质瘤发病相关蛋白1基因对香烟烟雾暴露小鼠肺部菌群的影响  

Effects of GLI pathogenesis-related 1 gene on lung microbiome of mice exposed to cigarette smoke

作　　者：朱文思 彭文珺 韩林晓 吴圆圆 周建 ZHU Wen-si;PENG Wen-jun;HAN Lin-xiao;WU Yuan-yuan;ZHOU Jian(Department of Respiratory,Zhongshan Hospital,Fudan University,Shanghai Institute of Respiratory Diseases,Shanghai 200032,China)

机构地区：[1]复旦大学附属中山医院呼吸科,上海市呼吸病研究所,上海200032

出　　处：《中国临床医学》2023年第2期238-244,共7页Chinese Journal of Clinical Medicine

基　　金：国家自然科学基金(81770039,82070045,82270040).

摘　　要：目的探讨胶质瘤发病相关蛋白1(GLI pathogenesis-related 1,GLIPR1)基因对香烟烟雾暴露小鼠的肺部菌群的影响。方法通过被动吸入二手烟构建香烟烟雾暴露COPD小鼠模型,将C57BL/6小鼠和GLIPR1基因敲除小鼠随机分为野生对照组(WT组)、野生型香烟烟雾暴露组(WT_SMOKE组)、GLIPR1基因敲除对照组(GLIPR1组)和GLIPR1基因敲除香烟烟雾暴露组(GLIPR1_SMOKE组)。采用16S rRNA测序技术得出肺部菌群丰度及多样性信息;采用LEfSe分析各组小鼠肺部菌群的差异。结果4组小鼠肺部微生物群β多样性差异显著(PANOSIM=0.003),4组小鼠肺部微生物群间存在28个差异菌属。进一步LEfSe分析提示,与WT组相比,WT_SMOKE组布劳特氏菌占比减少0.25%[线性判别式分析(LDA)评分=3.1367 log10,P=0.006]。GLIPR1_SMOKE组双歧杆菌属(LDA评分=3.465 log10,P=0.042)和芽单胞杆菌门S0134_terrestrial_group(LDA评分=3.115 log10,P=0.041)丰度最高。结论香烟烟雾暴露后,GLIPR1基因敲除小鼠的肺部菌群分布与野生型小鼠存在差异,其中以布劳特氏菌、双歧杆菌属和芽单胞杆菌门S0134_terrestrial_group差异最明显。Objective To explore the effect of GLI pathogenesis-related 1(GLIPR1)gene on the lung microbiome of mice exposed to cigarette smoke.Methods Mice were exposed to cigarette smoke to established COPD mouse model.C57BL/6 mice and GLIPR1 konckout mice were randomly divided into 4 groups:wild-type control group(WT group),wild-type cigarette smoke exposure group(WT_SMOKE group),GLIPR1 gene knockout control group(GLIPR1 group),and GLIPR1 gene knockout cigarette smoke exposure group(GLIPR1_SMOKE group).Then 16S rRNA sequencing technology was used to analyze the differences among the lung microbiome of mice in 4 groups.Results There were significant differences inβdiversity among the lung microbiota of 4 groups(PANOSIM=0.003).At the genus level,there were 28 differential bacterial genera among the 4 groups.LEfSe analysis showed that compared with WT group,the proportion of Blautia in mice of WT_SMOKE group decreased by 0.25%(LDA score=3.137 log10,P=0.006).The proportion of Bifidobacterium(LDA score=3.465 log10,P=0.042)and Gemmatimonadetes.S0134_terrestrial_group(LDA score=3.115 log10,P=0.041)were highest in GLIPR1_SMOKE group,respectively.Conclusions After exposure to cigarette smoke,the distribution of lung microbiome in GLIPR1 gene knockout mice is higher than that in WT mice,and Blautia,Bifidobacterium and Gemmatimonadetes.S0134_terrestrial_group play important roles.

关 键 词：肺部菌群 慢性阻塞性肺疾病 胶质瘤发病相关蛋白1 16S rRNA 

分 类 号：R563.9[医药卫生—呼吸系统]