Modeling cell contractility responses to acoustic tweezing cytometry  

作　　者：Suyan Zhang Zhenzhen Fan 

机构地区：[1]Department of Biomedical Engineering,Tianjin University,Tianjin 300072,China [2]State Key Laboratory of Acoustics,Institute of Acoustics,Chinese Academy of Sciences,Beijing 100190,China

出　　处：《Theoretical & Applied Mechanics Letters》2023年第2期132-142,共11页力学快报（英文版）

基　　金：This work is supported by the National Natural Science Founda-tion of China(Grant No.11874280);the State Key Laboratory of Acoustics,Chinese Academy of Sciences(Grant No.SKLA202211).

摘　　要：Acoustic tweezing cytometry(ATC)is a recently developed method for cell mechanics regulation.Tar-geted microbubbles,which are attached to integrins and subsequently the actin cytoskeleton,anchor,amplify and transmit the mechanical energy in an acoustic field inside the cells,eliciting prominent cy-toskeleton contractile force increases in various cell types.We propose that a mechanochemical con-version mechanism is critical for the high efficiency of ATC to activate cell contractility responses.Our models predict key experimental observations.Moreover,we study the influences of ATC parameters(ul-trasound center frequency,pulse repetition frequency,duty cycle,and acoustic pressure),cell areas,the number of ATC stimuli,and extracellular matrix rigidity on cell contractility responses to ATC.The simu-lation results suggest that it is large molecules,rather than small ions,that facilitate global responses to the local ATC stimulation,and the incorporation of visible stress fiber bundles improves the accuracy of modeling.

关 键 词：Cell contractility Cytoskeleton contractile force Acoustic tweezing cytometry 2D dynamic modeling 

分 类 号：Q2-33[生物学—细胞生物学]