活血益气方对miR-484过表达脐静脉内皮细胞增殖、迁移和成管能力的影响  

Effect of Huoxue Yiqi recipe on proliferation,migration and tube formation of miR⁃484 overexpressed umbilical vein endothelial cells

作　　者：杨睿 程序 赵梦竹 国倩倩 张馨月 刘梦华 魏琼 张冬梅[1] YANG Rui;CHENG Xu;ZHAO Meng-zhu;GUO Qian-qian;ZHANG Xin-yue;LIU Meng-hua;WEI Qiong;ZHANG Dong-mei(Dongzhimen Hospital,Ministry of Education and Beijing Key Laboratory of Traditional Chinese Medicine Internal Medicine,Beijing University of Chinese Medicine,Beijing 100700,China)

机构地区：[1]北京中医药大学东直门医院/中医内科学教育部和北京市重点实验室,北京100700

出　　处：《海南医学院学报》2023年第9期660-665,673,共7页Journal of Hainan Medical University

基　　金：国家自然科学基金项目(81973780)。

摘　　要：目的:探讨活血益气方通过调节miR-484靶向VEGF调节血管新生的可能分子机制。方法:制备miR-484过表达HUVEC细胞模型,采用活血益气方药物冻干粉干预,CCK-8法、细胞划痕实验、Matrigel体外三维成形实验分别检测活血益气方对HUVEC增殖、迁移和管状成形能力的影响,实时荧光定量PCR法检测活血益气方对VEGFA mRNA表达的影响。结果:与对照组相比,miR-484过表达模型组细胞增殖能力减弱(P<0.01),迁移面积减少(P<0.05),参与成管的长度、分支数、节点数减少,成管能力显著下降(P<0.01),VEGFA mRNA表达水平下调(P<0.05);与模型组相比,活血益气方药物组细胞增殖能力增强(P<0.01),迁移面积增加(P<0.05),参与成管的长度、分支数、节点数增多,成管能力提升(P<0.05),VEGFA mRNA表达水平上调(P<0.05)。结论:miR-484抑制VEGFA mRNA的表达和HUVEC的增殖、迁移和成管,活血益气方对该过程有一定的负向调控作用,这可能是活血益气方促进血管新生的新的作用靶点。Objective:To explore the possible mechanism of Huoxue Yiqi recipe promoting angiogenesis by targeting VEGF with miR‑484.Methods:The model of miR‑484 overexpressed in HUVEC was prepared and intervened with Huoxue Yiqi Recipe Freeze‑dried powder.The effects of Huoxue Yiqi Recipe on proliferation,migration and tubular forming ability of HUVEC were separatly detected by CCK‑8 method,cell scratch test and Matrigel three‑dimensional forming test in vitro.The effect of Huoxue Yiqi Recipe on VEGFA mRNA expression was detected by real‑time fluorescence quantitative PCR.Results:Compared with the control group,the proliferation and migration area of cells in miR‑484 overexpression model group decreased,the length,number of branches and nodes involved in tube formation decreased,the tube formation ability decreased significantly,and the expression level of VEGFA mRNA decreased,with statistical significance.Compared with the model group,the cell proliferation ability,migration area,length,number of branches and nodes involved in tube formation,tube formation ability and expression level of VEGFA mRNA in the Huoxue Yiqi Prescription group were up‑regulated,and the difference was statistically significant(P<0.05).Conclusion:MiR‑484 inhibits the expression of VEGFA and the proliferation,migration and formation of HUVEC,and Huoxue Yiqi recipe has a certain negative regulation effect on this process,which may be a new target of Huoxue Yiqi recipe to promote angiogenesis.

关 键 词：miR-484 活血益气方 血管新生 VEGF 心肌梗死 

分 类 号：R285[医药卫生—中药学]