槟榔种质资源离体繁殖体系研究  

Study on in Vitro Reproductive Technical System of Areca-nut(Areca catechu L.)Germplasm Resources

作　　者：李栋梁 王景飞 周颖怡 陈业光 张雯婷 戚华沙 陈宣 LI Dongliang;WANG Jingfei;ZHOU Yingyi;CHEN Yeguang;Zhang Wenting;QI Huasha;CHEN Xuan(Hainan Provincial Key Laboratory for Innovation and Utilization of Tropical Special Economic Plants,Institute of Tropical Horticulture,Hainan Academy of Agricultural Sciences,Haikou,Hainan 571100;Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine,Hainan Branch,the Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences,Haikou,Hainan 570311,China)

机构地区：[1]海南省农业科学院热带园艺研究所,海南省热带特种经济植物种质资源创新利用重点实验室,海南海口571100 [2]中国医学科学院药用植物研究所海南分所,海南省南药资源保护与开发重点实验室,海南海口570311

出　　处：《贵州农业科学》2023年第5期85-90,共6页Guizhou Agricultural Sciences

基　　金：海南省重点研发计划项目(ZDYF2022XDNY162);海南省农业科学院基本科研业务费专项项目“槟榔种质资源调查、收集与初步评价”(No.27);海南省乡村振兴及百镇千村补助资金项目“道地药材良种繁育和生态种植基地建设”(2022)。

摘　　要：【目的】建立槟榔离体繁殖技术体系,为保护和保存槟榔优良种质资源及资源创新利用提供技术支撑。【方法】以成熟健康的槟榔果实为材料,探索不同消毒时间、不同激素浓度的MS培养基对芽的诱导、增殖和生根的影响。【结果】将成熟健康的槟榔果实浸泡在100 mg/L IAA 24 h后,去皮取种子置于0.1%HgCl_(2)浸泡10 min消毒效果最佳,污染率为10%,萌芽率达94.75%;槟榔芽诱导最优激素组合培养基为MS+3.00 mg/L 6-BA+0.5 mg/L NAA,萌芽率最高达95.67%;将出芽材料转接于MS+1.0 mg/L NAA培养基中诱导生根,生根率和生根数分别达95.33%和7.33条。【结论】建立的槟榔种质资源离体繁殖技术操作性强、可行性高,为槟榔离体资源库的建立与开发利用及可持续发展奠定了基础。【Objective】The in vitro reproductive technological system of areca-nut(Areca catechu L.)is established to provide the technical support for protection,preservation,innovation and utilization of excellent areca-nut germplasm resources.【Method】The effects of different sterilization time and MS medium with different concentration of hormones on bud induction,propagation and rooting of the mature and healthy acera-nuts are studied.【Result】The acera-nuts are firstly soaked in 100 mg/L IAA for 24 hours to remove the peel.The contamination rate and bud germination rate of the acera-nuts without peel soaked in 0.1%HgCl_(2)for 10 min reach 10%and 94.75%respectively.The bud germination rate of the disinfectant acera-nuts cultured on MS+3.00 mg/L 6-BA+0.5 mg/L NAA can reach 95.67%.The rooting rate and rooting number of acera nut buds cultured on MS+1.0 mg/L NAA are 95.33%and 7.33 separately.【Conclusion】The established in vitro reproductive technical system of acera-nut germplasm resources with the advantages of easy operability and higher feasibility can lay a foundation for establishment,exploitation,utilization and sustainable development of in vitro acera-nut germplasm resources.

关 键 词：槟榔 种质资源 外植体 离体培养 消毒效果 萌芽率 

分 类 号：S763.7[农业科学—森林保护学]