电针对逼尿肌过度活动大鼠膀胱ICCs相关HCN亚型及钙离子震荡特性的影响  

Impact of electroacupuncture on ICCs-associated HCN subtypes and calcium ion oscillation features in the bladder of detrusor overactivity rats

作　　者：张安冬[1] 冯戟玮 刘建党 李雯 谢旭彬 陈跃来 ZHANG Andong;FENG Jiwei;LIU Jiandang;LI Wen;XIE Xubin;CHEN Yuelai(Yueyang Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200437,China;Longhua Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 200032,China;Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Shanghai Seventh People’s Hospital,Shanghai 200137,China)

机构地区：[1]上海中医药大学附属岳阳中西医结合医院,上海200437 [2]上海中医药大学附属龙华医院,上海200032 [3]上海中医药大学,上海201203 [4]上海市第七人民医院,上海200137

出　　处：《上海针灸杂志》2023年第5期535-542,共8页Shanghai Journal of Acupuncture and Moxibustion

基　　金：国家自然科学基金项目(81674090)。

摘　　要：目的观察电针对逼尿肌过度活动(detrusor overactivity,DO)大鼠膀胱Cajal间质细胞(interstitial cells of cajal,ICCs)超极化激活环核苷酸门控阳离子通道(hyperpolarization-activated cyclic nucleotide-gated cation channels,HCN)相关亚型表达水平及细胞内钙离子震荡特性的影响。方法40只Wistar雌性大鼠随机分为对照组、模型组、电针组和药物组,每组10只。除对照组外,其余大鼠均采用环磷酰胺腹腔注射制备DO模型,电针组在模型基础上给予电针次髎和会阳,每日1次,每次20 min,连续3 d;药物组予甲磺酸伊马替尼灌胃,每日1次,连续3 d。对照组、模型组和药物组大鼠同电针组予以对照捆绑固定20 min,对照、模型和电针组同药物组予以同等体质量计量的饮用水灌胃处理,均每日1次,连续3 d。采用Western Blot和RT-PCR检测HCN1-44种亚型的表达,运用钙离子成像技术检测电针对ICCs内钙离子震荡特性的影响。结果与对照组比较,模型组大鼠膀胱ICCs相关HCN1和HCN2的蛋白和mRNA表达均升高(P<0.05);与模型组比较,电针组和药物组HCN1和HCN2的蛋白和mRNA表达均降低(P<0.05);4组HCN3、HCN4比较差异均无统计学意义(P>0.05)。与对照组比较,模型组大鼠膀胱ICCs内钙离子震荡幅度、频率和宽度明显升高(P<0.05);与模型组比较,电针组和药物组膀胱ICCs内钙离子震荡频率和幅度均下降(P<0.05),而钙离子震荡宽度无明显变化(P>0.05)。结论电针可能通过抑制DO大鼠膀胱ICCs起搏通道相关HCN1和HCN2的表达,及降低ICCs内钙离子震荡的幅度和频率,达到调控DO的作用。Objective To observe the effects of electroacupuncture(EA)on the expression levels of the subtypes of hyperpolarization-activated cyclic nucleotide-gated cation channels(HCN)and intracellular calcium ion oscillation characteristics of the interstitial cells of Cajal(ICCs)in rats with detrusor overactivity(DO).Method Forty female Wistar rats were randomized into a control group,a model group,an EA group,and a medication group,with 10 rats in each group.Except for the control group,rats in the other groups underwent DO modeling with an intraperitoneal injection of Cyclophosphamide;besides,the EA group was given EA at Ciliao(BL32)and Huiyang(BL35)points,20 min each time,once daily for 3 consecutive days,and the medication group received intragastric administration of Imatinib mesylate,once daily for 3 consecutive days.Rats in the control,model,and medication groups were fixed for 20 min as those in the EA group,and rats in the control,model,and EA groups received intragastric administration of drinking water with the amount calculated based on the body mass just in the same way as in the medication group,once daily for 3 successive days.Western Blot and RT-PCR were adopted to detect the expression of HCN1-4 subtypes,and the impact of EA on the features of ICCs intracellular calcium ion oscillation was examined using calcium imaging technology.Result Compared with the control group,the protein and mRNA expression levels of ICCs-associated HCN1 and HCN2 in the bladder of model group rats increased(P<0.05);compared with the model group,the protein and mRNA expression levels of HCN1 and HCN2 decreased in the EA and medication groups(P<0.05);there were no significant differences in the expression of HCN3 or HCN4 among the four groups(P>0.05).Compared with the control group,the bladder ICCs intracellular calcium ion oscillation amplitude,frequency,and width were heightened in the model group rats(P<0.05);compared with the model group,the bladder ICCs intracellular calcium ion oscillation amplitude and frequency dropped in

关 键 词：电针 逼尿肌过度活动 CAJAL间质细胞 超极化激活环核苷酸门控阳离子通道 大鼠 

分 类 号：R2-03[医药卫生—中医学]