禽致病性大肠杆菌clpV基因缺失对雏鸡气管黏膜细胞因子-细胞因子受体相互作用通路的影响  

Effect of avian pathogenic Escherichia coli clpV gene deletion on the cytokine-cytokine receptor interaction pathway in chick tracheal mucosa

作　　者：蒋胡艳 肖福泉 龚柳菲 薛新颖 邵颖[1] 涂健[1] 祁克宗[1] 宋祥军 JIANG Huyan;XIAO Fuquan;GONG Liufei;XUE Xinying;SHAO Ying;TU Jian;QI Kezong;SONG Xiangjun(School of Animal Science and Technology,Anhui Agricultural University,Hefei 230036)

机构地区：[1]安徽农业大学动物科技学院,合肥230036

出　　处：《安徽农业大学学报》2023年第2期255-260,共6页Journal of Anhui Agricultural University

基　　金：安徽省大学生创新创业训练计划项目(S202110364034);安徽省大学生创新创业训练计划项(202110364027)共同资助;国家级大学生创新创业训练计划项目(202110364009)。

摘　　要：为探究clpV基因在禽致病性大肠杆菌(Avian pathogenic Escherichia coli,APEC)感染雏鸡气管黏膜过程中发挥的作用及机制。将APEC野生株(DE17)及其基因缺失株(DE17ΔclpV)感染7日龄雏鸡气管,12、24h后收集雏鸡气管黏膜细胞进行转录组学测序,筛选野生株和缺失株的差异表达基因,进行GO和KEGG富集分析。结果显示,在感染12 h后,共筛选到108个差异表达基因(36个上调,72个下调);在感染24 h后,共筛选到59个差异表达基因(34个上调,25个下调)。GO分析表明,差异表达基因主要富集在细胞内信号转导、RNA聚合酶Ⅱ的转录正调控等生物学过程;蛋白质结合、ATP合成、DNA结合等分子功能类;生物膜及膜的组成成分、细胞质等细胞组分功能;KEGG分析表明,差异表达基因主要富集在紧密连接通路、内质网中的蛋白质加工通路、细胞因子与细胞因子受体相互作用通路、内吞通路、Wnt信号通路等。结论为clpV基因缺失后,会使部分差异表达基因的表达量下调,对细胞因子-细胞因子受体相互作用等通路产生影响。To investigate the role and mechanism of the clpV gene in the process of Avian pathogenic Escherichia coli(APEC)infection of the chicks racheal mucosa.In this study,7-day-old chicks were infected with the wild strain(DE17)and the deletion strain(DE17ΔclpV)of APEC,and the chicks'tracheal mucosa cells were collected 12 and 24 h later for transcriptomic sequencing to screen the differentially expressed genes of the wild strain and the deletion strain.The results showed that a total of 108 differentially expressed genes(36 up-regulated and 72 down-regulated)were screened 12 h after infection and 59 differentially expressed genes(34 up-regulated and 25 down-regulated)were screened 24 h after infection.GO analysis showed that the differentially expressed genes were mainly enriched in biological processes such as intracellular signal transduction,positive regulation of RNA polymerase Ⅱ transcription;protein binding,ATP synthesis,DNA binding and other molecular functional classes;cellular component functions such as biofilm and membrane components,cytoplasm.KEGG analysis showed that the differentially expressed genes were mainly enriched in the tight junction pathway,protein processing pathway in the endoplasmic reticulum,cytokine-cytokine receptor interaction pathway,endocytosis pathway and Wnt signalling pathway,etc.It was concluded that deletion of the clpV gene resulted in the down-regulation of the expression of some of the differentially expressed genes,with effects on pathways such as cytokine-cytokine receptor interactions.

关 键 词：禽致病性大肠杆菌 VI型分泌系统 clpV基因 转录组学测序 气管黏膜细胞 

分 类 号：S852.612[农业科学—基础兽医学]