真菌诱导子对白术悬浮细胞生长与次生代谢的影响  

Effects of Fungal Elicitors on Growth and Secondary Metabolism of Atractylodes macrocephala Suspension Cells

作　　者：孙天驰 董诗慧 余佳琰 裘芳琼 秦路平 朱波[1] SUN Tianchi;DONG Shihui;YU Jiayan;QIU Fangqiong;QIN Luping;ZHU Bo(School of Pharmaceutical Sciences,Zhejiang Chinese Medical University,Hangzhou 311402,China)

机构地区：[1]浙江中医药大学药学院,浙江杭州311402

出　　处：《河南农业科学》2023年第4期60-65,共6页Journal of Henan Agricultural Sciences

基　　金：国家自然科学基金项目(82003896);浙江省自然科学基金项目(LQ21H280003)。

摘　　要：为探讨不同真菌诱导子对白术悬浮细胞生长和次生代谢的影响,于无菌状态下培养白术无菌苗,诱导愈伤组织,并构建悬浮细胞培养体系,记录悬浮细胞干质量,绘制生长曲线,添加不同内生真菌诱导子溶液后共培养,用高效液相色谱法测定悬浮细胞中白术内酯Ⅰ、白术内脂Ⅲ与苍术酮含量。结果表明,MS+6-BA(6-苄氨基嘌呤)1.0 mg/L+NAA(1-萘乙酸)0.5 mg/L是白术愈伤组织诱导的最适培养基,MS+6-BA 0.5 mg/L+NAA 0.5 mg/L是悬浮细胞增殖的最适培养基;白术悬浮细胞生长呈现S形曲线生长特征,对数生长期培养体系pH值稳定在4.8~5.0;真菌诱导子显著影响悬浮细胞生长与次生代谢,其中AM478真菌诱导子和AM393真菌诱导子对悬浮细胞生长具有显著促进作用,AM569真菌诱导子对白术内酯Ⅲ与苍术酮积累具有显著促进作用。综上,筛选得到的AM569菌诱导子可作为白术悬浮细胞生物转化的备选诱导子,以促进白术有效成分积累。This study aimed to explore the effects of different fungal elicitors on growth and secondary metabolism of Atractylodes macrocephala(AM)suspension cells.First,AM seedlings were cultured in a sterile state to induce calluses and prepare suspension cells,and the cell dry mass was recorded.Then,various elicitors were added to the suspension cell culture system,and atractylideⅠ,atractylideⅢand atractylone contents were determined using high performance liquid chromatography(HPLC)technology.The results showed that MS medium+6‑BA(6‑benzylaminopurine)1.0 mg/L+NAA(1‑naphthaleneacetic acid)0.5 mg/L was the optimal medium for the induction of calluses,while MS medium+6‑BA 0.5 mg/L+NAA 0.5 mg/L was the optimal medium for the proliferation of suspended cells.The growth of AM suspension cells showed S‑shaped curve growth characteristics,and the pH of the culture system in the logarithmic growth phase ranged between 4.8 and 5.0.Fungal elicitors significantly promoted the growth and secondary metabolism of AM suspension cells,among which AM478 elicitor and AM393 elicitor showed the best growth promotion activity.In addition,AM569 elicitor was found to significantly promote the accumulation of atractylodesⅢand atractylone of AM suspension cells.In conclusion,AM569 fungal elicitor can be used as an alternative for the biotransformation of AM suspension cells to promote the accumulation of active components of AM.

关 键 词：真菌诱导子 白术 悬浮细胞 生长 次生代谢 

分 类 号：S567.19[农业科学—中草药栽培]