“微-纳”PCL纤维支架多级结构对巨噬细胞极化状态的调控  

Regulation of macrophage polarization by micro-nano multilevel structure of electrospun PCL fibrous scaffolds

作　　者：张荣妍 李晓婧 李超婧 王富军[1,2] 王璐[1,2] ZHANG Rongyan;LI Xiaojing;LI Chaojing;WANG Fujun;WANG Lu(College of Textiles,Ministry of Education,Donghua University,Shanghai 201620,Chin;Key Laboratory of Textile Science&Technology,Ministry of Education,Donghua University,Shanghai 201620,China)

机构地区：[1]东华大学纺织学院,上海201620 [2]东华大学纺织面料技术教育部重点实验室,上海201620

出　　处：《东华大学学报（自然科学版）》2023年第2期1-8,23,共9页Journal of Donghua University(Natural Science)

基　　金：上海市青年科技英才扬帆计划资助项目(20YF1400800);中央高校基本科研业务费专项资金资助项目(2232020G-01);111计划2.0(BP0719035)。

摘　　要：为探究纤维基材料的孔径和表面形貌对巨噬细胞极化表型的影响,结合静电纺丝技术和溶液诱导结晶法制备不同孔径的微-纳多级结构聚己内酯(polycaprolactone,PCL)纤维支架。通过微观形貌分析及水接触角、拉伸性能和生物性能测试分别评价支架的形貌、润湿性、力学性能及其调控的巨噬细胞极化表型。结果表明:大直径的纤维支架比小直径的纤维支架的孔径大、刚度低,更有利于巨噬细胞向M2型极化(抑炎表型)。相比光滑的纤维支架,具有串晶结构的大孔径纤维支架能够促进巨噬细胞分泌抑炎细胞因子IL-4,并减少促炎因子IL-6的分泌。In order to explore the effect of pore size and surface topography of fiber-based biomaterials on macrophage polarization,polycaprolactone(PCL)fibrous scaffolds with micro-nano multilevel structure and variable pore sizes were prepared by electrospinning combined with solution-induced crystallization method.The topography,surface wettability,mechanical properties and macrophage phenotype of the scaffolds were tested by microtopography analysis,tensile test,water contact angle test and biological properties test.The as-prepared fibrous scaffolds with larger fiber diameter had larger pore size and lower stiffness than the smaller one,and effectively stimulated macrophages towards M2(antiinflammatory phenotype).Moreover,compared with smooth fibrous scaffolds,the larger pore size fibrous scaffolds with shish-kebab structure promoted production of anti-inflammatory cytokine IL-4 and reduced production of pro-inflammatory cytokine IL-6 released by macrophages.

关 键 词：巨噬细胞极化 静电纺纤维支架 孔径 串晶结构 

分 类 号：TS101.4[轻工技术与工程—纺织工程]