肛周脓肿组织SBP1、IL-17表达与患者临床病理特征及预后的关系  

The relationship between the expression of SBP1 and IL-17 in perianal abscess tissue and the clinical pathological characteristics and prognosis of patients

作　　者：罗蕾 黄鸿翔 庄怡青[1] 唐英 Luo Lei;Huang Hongxiang;Zhuang Yiqing;Tang Ying(Day-Care Unit,Xinhua Hospital Affiliated to Medical College of Shanghai Jiaotong University,Shanghai 200092,China)

机构地区：[1]上海交通大学医学院附属新华医院日间病房,200092

出　　处：《疑难病杂志》2023年第5期520-525,530,共7页Chinese Journal of Difficult and Complicated Cases

基　　金：上海市卫生健康委员会卫生行业临床研究专项项目(202140510)。

摘　　要：目的分析肛周脓肿组织硒结合蛋白1(SBP1)、白介素-17(IL-17)表达与患者临床病理特征及预后的关系。方法选取2020年1月—2021月12月上海交通大学医学院附属新华医院日间病房手术治疗肛周脓肿患者120例作为肛周脓肿组,并根据6个月预后情况分为预后良好亚组75例和预后不良亚组45例。另选取同期收治的无感染混合痔患者120例作为对照组,留取2组病变组织标本,检测并比较2组患者局部病变组织中SBP1、IL-17 mRNA相对表达量及蛋白阳性表达率,分析肛周脓肿患者局部组织中SBP1、IL-17蛋白阳性表达率、mRNA相对表达量与临床病理特征的关系;Logistic多因素回归分析影响肛周脓肿患者预后的危险因素;采用受试者工作特征曲线(ROC)分析SBP1、IL-17 mRNA相对表达量对肛周脓肿患者预后的预测价值。结果肛周脓肿组SBP1 mRNA相对表达量及蛋白阳性率低于对照组[t(χ^(2))/P=30.059/<0.001,33.189/<0.001],IL-17 mRNA相对表达量及蛋白阳性率高于对照组[t(χ^(2))/P=29.003/<0.001,126.159/<0.001];预后不良亚组患者局部组织中SBP1 mRNA相对表达量、蛋白阳性率均低于预后良好亚组[t(χ^(2))/P=10.051/<0.001,6.012/0.014],而IL-17 mRNA相对表达量、蛋白阳性率均高于预后良好亚组[t(χ^(2))/P=5.597/0.018,4.474/<0.001]。肛周局部组织中SBP1、IL-17表达在不同患者性别、年龄、脓肿位置、感染类型、病程比较差异无统计学意义(P>0.05);与脓肿<5 cm比较,脓肿≥5 cm患者SBP1 mRNA相对表达量、蛋白阳性率降低[t(χ^(2))/P=3.630/<0.001,4.472/0.034],IL-17 mRNA相对表达量、蛋白阳性率升高[t(χ^(2))/P=4.387/<0.001,7.721/0.005];与术后愈合时间<4周比较,术后愈合时间≥4周患者SBP1 mRNA相对表达量、蛋白阳性率降低[t(χ^(2))/P=3.070/0.003,4.577/0.032],IL-17 mRNA相对表达量、蛋白阳性率显著升高[t(χ^(2))/P=5.768/<0.001,5.006/0.025]。Logistic多因素回归分析显示,术后愈合时间≥4周、SBPObjective To analyze the relationship between the expression of selenium binding protein 1(SBP1)and interleukin-17(IL-17)in perianal abscess tissue and the clinical pathological characteristics and prognosis of patients.Methods One hundred and twenty patients with perianal abscess who underwent surgical treatment in the daytime ward of Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine from January 2020 to December 2021 were selected as the perianal abscess group.Based on the prognosis after 6 months,they were divided into a subgroup of 75 patients with good prognosis and a subgroup of 45 patients with poor prognosis.Another 120 non-infected mixed hemorrhoid patients admitted at the same time were selected as the control group.Two groups of pathological tissue samples were collected,and the relative expression levels of SBP1 and IL-17 mRNA and protein positive expression rates in the local pathological tissues of the two groups of patients were detected and compared.The relationship between the positive expression rates of SBP1 and IL-17 protein,relative expression levels of mRNA and clinical pathological characteristics in the local tissues of patients with perianal abscess was analyzed;Logistic multiple factor regression analysis is used to identify the risk factors that affect the prognosis of patients with perianal abscess;The predictive value of relative expression levels of SBP1 and IL-17 mRNA on the prognosis of patients with perianal abscess was analyzed using the receiver operating characteristic curve(ROC).Results The relative expression level and protein positive rate of SBP1 mRNA in the perianal abscess group were lower than those in the control group[t(χ^(2))/P=30.059/<0.001,33.189/<0.001],the relative expression level of IL-17 mRNA and protein positivity rate were higher than those in the control group[t(χ^(2))/P=29.003/<0.001,126.159/<0.001];The relative expression level and protein positivity rate of SBP1 mRNA in local tissues of patients with poor prognosis subgroup

关 键 词：肛周脓肿 硒结合蛋白1 白介素-17 临床病理特征 预后 相关性 

分 类 号：R657.15[医药卫生—外科学]