低温胁迫对小粒种咖啡幼苗生理特性的影响  

Physiological and Biochemical Characteristics of Coffea arabica Seedling Under Low Temperature Stress

作　　者：黄丽芳[1,2] 龙宇宙 李金芹[3] 董云萍 王晓阳[1] 陈鹏[1] 王宪文[4] 闫林 HUANG Lifang;LONG Yuzhou;LI Jinqin;DONG Yunping;WANG Xiaoyang;CHEN Peng;WANG Xianwen;YAN Lin(Spice and Beverage Research Institute,Chinese Academy of Tropical Agricultural Science,Key Laboratory of Genetic Resources Utilization of Spice and Beverage Crops,Ministry of Agriculture and Rural Affairs,Key Laboratory of Genetic Improvement and Quality Regulation for Tropical Spice and Beverage Crops of Hainan Province,Hainan Wanning 571533,China;Hainan Sim Soonliang Academician Workstation,Hainan Wanning 571533,China;College of Tropical Crops,Yunnan Agricultural University,Yunnan Puer 665099,China;Baoshan Comprehensive Inspection Center of Quality and Technical Supervision,Yunnan Baoshan 678000,China)

机构地区：[1]中国热带农业科学院香料饮料研究所,农业农村部香辛饮料作物遗传资源利用重点实验室,海南省热带香辛饮料作物遗传改良与品质调控重点实验室,海南万宁571533 [2]海南省Sim Soonliang院士工作站,海南万宁571533 [3]云南农业大学热带作物学院,云南普洱665099 [4]保山市质量技术监督综合检测中心,云南保山678000

出　　处：《中国农业科技导报》2023年第2期60-67,共8页Journal of Agricultural Science and Technology

基　　金：海南省自然科学基金资助(322QN405);国家木薯产业技术体系项目(CARS-11);云南省科技厅院士(专家)工作站项目(202105AF150081)。

摘　　要：为探讨小粒种咖啡的耐寒机制,以1年生小粒种咖啡为试验材料,对不同低温过程中叶片渗透调节物质含量、抗氧化酶活性及细胞膜透性等生理指标进行测定,探究其变化规律,并对各个指标间的关系进行相关性及主成分分析。结果表明,小粒种咖啡叶片脯氨酸、可溶性糖、可溶性蛋白含量以及总超氧化物歧化酶和过氧化氢酶活性在8℃处理时平稳上升,在4、0、-2℃处理时均呈先升后降的趋势;过氧化物酶活性随着温度的降低和处理时间的延长也先升高后降低。丙二醛含量和相对电导率变化趋势相似,均随着温度降低逐渐增加,二者均在0℃处理48 h和-2℃处理48 h时达较大值,为132.00、124.58 nmol·g^(-1)和91.29%、98.35%,相关性分析表明丙二醛和相对电导率呈极显著正相关关系。综上所述小粒种咖啡在低温胁迫下通过调节自身的抗氧化酶活性及渗透调节物质含量来缓解低温胁迫造成的伤害,表现出较强的抗寒能力,该结果可为咖啡的资源保护、引种驯化及品种选育提供理论支持。To investigate the mechanism of cold-resistance in Coffea arabica,a few physiological indexes including osmotic adjustment substance content,antioxidant enzyme activities,and cell membrane permeability for leaves of oneyear-old seedlings under different low temperatures were measured,and which were treated with correlations and principal component analysis.The results indicated that the contents of proline,soluble sugar,soluble protein,and the activities of superoxide dismutase and catalase were increased steadily at 8℃,while they were showed a trend of increasing first and then decreasing at 4,0 and-2℃.For peroxidase activities,they also had the same trends with decreasing of temperatures and extension of processing time.The malondialdehyde content and relative conductivity had similar trends,manifested as increasing gradually with temperature decreasing,and maximum values were both reached at 0℃for 48 h and-2℃for 48 h,which were 132.00 and 124.58 nmol·g^(-1),91.29%and 98.35%,respectively.Correlation analysis showed that there was a highly significant positive correlation between malondialdehyde content and relative conductivity.In summary,Coffea arabica seedlings could alleviate damage from low temperature stress and showed strong coldresistance ability by regulating its own antioxidant enzyme activities and osmotic adjustment substance contents.This study would provide theoretical support for resource protection,introduction,domestication and variety breeding of coffee.

关 键 词：小粒种咖啡 低温胁迫 生理指标 相关性 

分 类 号：S571.2[农业科学—作物学]