淫羊藿苷对阿霉素肾病大鼠肾损伤的影响及其协同激素增效机制的研究  

Study on effect of icariin on renal injury in adriamycin induced nephropathy rats and its synergistic mechanism

作　　者：王晓辉 戴恩来[1] 段淑文 刘灿 丁照然 孟保伟 Wang Xiao-hui;Dai En-lai;Duan Shu-wen;Liu Can;Ding Zhao-ran;Meng Bao-wei(Gansu University of Chinese medicine,Lanzhou,Gansu 730000,China;Department of Thoracic Surgery,Central Hospital of Gansu Province,Lanzhou,Gansu 730050,China)

机构地区：[1]甘肃中医药大学,甘肃兰州730000 [2]甘肃省中心医院胸外科,甘肃兰州730050

出　　处：《中国现代医学杂志》2023年第9期25-31,共7页China Journal of Modern Medicine

基　　金：国家自然科学基金(No:82160852)。

摘　　要：目的探究淫羊藿苷(ICA)通过炎症-纤维化反应对阿霉素肾病模型大鼠肾损伤的影响及其协同激素增效的作用机制。方法60只SD大鼠随机取12只作为空白组,其余48只复制阿霉素肾病模型,分为模型组、泼尼松组、ICA组及联合组,每组12只。泼尼松组按6.3 mg/(kg·d)剂量予以泼尼松,ICA组按50 mg/(kg·d)剂量予以ICA,联合组按泼尼松组和ICA组的药物剂量予以泼尼松+ICA,空白组和模型组仅予以0.9%生理盐水按1 mL/100 g剂量灌胃,并连续灌胃6周。全自动血液生化分析仪检测血清白蛋白(ALB)、尿素氮(BUN)、肌酐(Scr);酶联免疫吸附试验检测白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、肿瘤坏死因子α(TNF-α)、转化生长因子-β(TGF-β)、纤维黏连蛋白(FN);Western blotting检测SPARC蛋白表达;Masson染色法观察肾组织病理学改变。结果与空白组相比,模型组大鼠肾小球基底膜沉积物逐渐增多,肾小球硬化和肾小管间质纤维组织增生,广泛间质纤维化;与空白组相比,24 h尿蛋白量升高,ALB、Scr降低,BUN、IL-1β、IL-6、TNF-α、TGF-β、FN升高,SPARC蛋白相对表达量升高(P<0.05)。与模型组相比,各治疗组上述病理改变减轻,24 h尿蛋白量降低,ALB、Scr升高,BUN、IL-1β、IL-6、TNF-α、TGF-β、FN降低,SPARC蛋白相对表达量下降(P<0.05)。结论ICA能够减轻炎症反应,抑制纤维化,对阿霉素肾病大鼠肾损伤发挥保护作用。Objective To investigate the effect of icariin on renal injury in adriamycin induced nephropathy rats through inflammation fibrosis reaction and its mechanism.Methods Sixty SD rats were randomly divided into model group,prednisone group,ICA group,and combination group,with 12 rats in each group.Prednisone group was given prednisone 6.3 mg/(kg·d),ICA group was given icariin 50 mg/(kg·d),combined group(prednisone+icariin),the blank group,and model group were given 0.9%normal saline 1ml/100g by gavage for 6 weeks.Serum albumin(ALB),urea nitrogen(BUN),and creatinine(Scr)were detected by automatic blood biochemical analyzer,and IL-1 was detected by enzyme-linked immunosorbent assay IL-1β,IL-6,TNF-α,TGF-β,and FN.The expression of SPARC protein was detected by Western blot,and the renal histopathological changes were observed by Masson staining.Results Compared with the blank group,the deposits of glomerular basement membrane in the model group increased gradually,glomerulosclerosis,proliferation of tubulointerstitial fibrous tissue,and extensive interstitial fibrosis were observed;Weight loss,24 h urine protein increase,ALB,Scr decrease,BUN,IL-1β,IL-6,TNF-α,TGF-β,and FN increased(P<0.05);SPARC protein expression increased(P<0.05).Compared with the model group,the above pathological changes in each treatment group were reduced,the body weight increased,the 24-hour urine protein decreased,ALB,Scr increased,BUN,IL-1β,IL-6,TNF-α,TGF-β,FN,and SPARC protein expression decreased(P<0.05).Conclusion ICA can reduce inflammatory reaction,inhibit fibrosis,and play a protective role on renal injury in rats with adriamycin nephropathy.

关 键 词：阿霉素肾病 淫羊藿苷 炎症 纤维化 肾损伤 

分 类 号：R692[医药卫生—泌尿科学]