植物乳杆菌HFY09通过调控TGF-β1表达对小鼠狼疮性肾炎的干预作用  

Intervention effect of Lactobacillus plantarum HFY09 on lupus nephritis in mice by regulating TGF-β1 expression

作　　者：易若琨 赵欣[2] 刘佳 杨贞耐[1] YI Ruokun;ZHAO Xin;LIU Jia;YANG Zhennai(Key Laboratory of Geriatric Nutrition and Health of Ministry of Education,Beijing Technology and Business University,Beijing 100048,China;Collaborative Innovation Center for Child Nutrition and Health Development,Chongqing University of Education,Chongqing 400067,China)

机构地区：[1]北京工商大学老年营养与健康教育部重点实验室,北京100048 [2]儿童营养与健康发展创新中心(重庆第二师范学院),重庆400067

出　　处：《食品与发酵工业》2023年第9期32-39,共8页Food and Fermentation Industries

基　　金：重庆市高校创新研究群体项目(CXQTP20033)。

摘　　要：该研究观察了一株分离于自然发酵牦牛酸乳的植物乳杆菌HFY09(Lactobacillus plantarum HFY09,LP-HFY09),通过降植烷建立狼疮性肾炎动物模型验证了LP-HFY09的作用,并通过试剂盒法、苏木精-伊红染色法、qPCR和Western blot检测小鼠血清和肾组织的相关指标。实验结果显示,LP-HFY09能够降低狼疮性肾炎造成的尿蛋白升高和血清、肾组织白介素6(interleukin-6,IL-6)、IL-12、TNF-α和IFN-γ细胞因子水平升高。LP-HFY09还可以降低肾炎小鼠血清肌酐、尿素氮、总胆固醇、甘油三酯水平和提高总蛋白、白蛋白水平。另外LP-HFY09具有抑制肾炎小鼠抗双链DNA抗体阳性率的作用。切片观察发现LP-HFY09能够减轻肾炎造成的肾小球形态不完整和炎性浸润等组织损伤。LP-HFY09还能够下调狼疮性肾炎小鼠肾脏组织中的转化生长因子-β1(transforming growth factor-β1,TGF-β1)和血管内皮生长因子mRNA和蛋白表达和上调铜锌超氧化物歧化酶和锰超氧化物歧化酶表达。结果表明,LP-HFY09能够对狼疮性肾炎起到明显的干预作用,且LP-HFY09的作用与其浓度呈正相关,在109 CFU/kg作用下效果接近药物泼尼松。In this study,a strain of Lactobacillus plantarum HFY09(LP-HFY09)isolated from naturally fermented yak yoghurt was observed,and an animal model of lupus nephritis was established using pristane to verify the effect of LP-HFY09.The related indexes of mouse serum and kidney tissue were detected by kit method,hematoxylin-eosin staining(H&E)method,quantitative polymerase chain reaction(qPCR)and Western blot.The experimental results showed that LP-HFY09 could reduce the increase of urinary protein and the increase of serum and renal tissue interleukin-6(IL-6),IL-12,tumor necrosis factor alpha(TNF-α),interferonγ(IFN-γ)cytokine levels caused by lupus nephritis.LP-HFY09 could also reduce serum creatinine(SCr),blood urea nitrogen(BUN),total cholesterol(TC),triglyceride(TG)levels and increase total protein(TP),albumin(ALB)levels in nephritis mice.In addition,LP-HFY09 could inhibit the positive rate of anti-double-stranded deoxyribonucleic acid(dsDNA)in mice with nephritis.Section observation showed that LP-HFY09 could reduce tissue damage such as incomplete glomerular morphology and inflammatory infiltration caused by nephritis.LP-HFY09 can also down-regulate mRNA and protein expressions of transforming growth factor-β1(TGF-β1),vascular endothelial growth factor(VEGF)and Cu/Zn-SOD,Mn-SOD expressions in the kidney tissue of lupus nephritis mice.These results indicated that LP-HFY09 could play a significant role in interfering with lupus nephritis,and the effect of LP-HFY09 was positively correlated with its concentration,and its effect was close to that of the drug prednisone under the action of 109 CFU/kg.

关 键 词：狼疮性肾炎 降植烷 转化生长因子-β1(TGF-β1) 泼尼松 表达 

分 类 号：R593.242[医药卫生—内科学]