用于敌敌畏高灵敏检测的炭气凝胶@纳米金/离子液体修饰的乙酰胆碱酯酶传感器  

High sensitive detection of dichlorvos by acetylcholinesterase sensor based on carbon aerogel@gold nanomaterials/ionic liquid

作　　者：李晓倩[1] 邵征 LI Xiaoqian;SHAO Zheng(Puyang Vocational and Technical College,Puyang 457000,China;Changyuan Cooking Vocational and Technical College,Xinxiang 453400,China)

机构地区：[1]濮阳职业技术学院,河南濮阳457000 [2]长垣烹饪职业技术学院,河南新乡453400

出　　处：《食品与发酵工业》2023年第9期164-170,共7页Food and Fermentation Industries

基　　金：河南省高等学校重点科研项目(22B550014);2020年度河南省高等职业学校青年骨干教师培养计划项目(2020GZGG113)。

摘　　要：在炭气凝胶的基础上制备氮掺杂炭气凝胶并负载金纳米棒,制备了新型的氮掺杂炭气凝胶@金纳米棒纳米复合材料(nitrogen doped carbon aerogel@gold nanorods,N-CA@Au)。以硼掺杂金刚石薄膜电极(boron-doped diamond,BDD)为工作电极,N-CA@Au和1-(4-磺酸)丁基-3-甲基咪唑硫酸氢盐离子液体(1-(4-sulfonic acid)butyl-3-methylimidazole bisulfate ionic liquid,[BSMIM]HSO_(4))为修饰材料,制备乙酰胆碱酯酶(acetylcholinesterase,AChE)传感器AChE/N-CA@Au-[BSMIM]HSO_(4)/BDD对蔬菜中的敌敌畏进行定量分析。研究发现,N-CA@Au具有良好的导电性和吸附特性,[BSMIM]HSO_(4)可以为AChE/N-CA@Au-[BSMIM]HSO_(4)/BDD提供稳定的电化学反应环境,使AChE、N-CA@Au和BDD发挥良好的协同作用,有效提高传感器的灵敏度。在最佳试验条件下敌敌畏浓度与其对AChE/N-CA@Au-[BSMIM]HSO_(4)/BDD抑制率的负对数在0.5×10^(-10)~1.0×10^(-5) g/L范围内呈线性关系,检出限为(按抑制率10%计算)1.9674×10^(-11) g/L,线性范围较宽,检出限较低,且该传感器稳定性和再生性较好,对常见重金属离子有很好的抗干扰能力。AChE/N-CA@Au-[BSMIM]HSO_(4)/BDD操作简单、方便、快捷可用于敌敌畏的快速定量分析。Nitrogen doped carbon aerogel(N-CAs)was prepared based on carbon aerogels and gold nanorods(Au)was loaded on N-CAs for a new nitrogen doped carbon aerogel@gold nanorods(N-CA@Au).Acetylcholinesterase chloride(AChE)sensor AChE/N-CA@Au-[BSMIM]HSO_(4)/BDD was prepared for detecting dichlorvos in vegetables using boron doped diamond film electrode(BDD)as working electrode,N-CA@Au and 1-(4-sulfonic acid)butyl-3-methylimidazole bisulfate ionic liquid([BSMIM]HSO_(4))as modified materials.The study found that N-CA@Au had a good conductivity and adsorption characteristics,[BSMIM]HSO_(4)could provide a stable electrochemical reaction environment for AChE/N-CA@Au-[BSMIM]HSO_(4)/BDD and make AChE,N-CA@Au and BDD play a good synergy,which can effectively improve the sensitivity of the sensor.Under the optimum test conditions,there was a linear relationship between the concentration of dichlorvos and the negative logarithm of the inhibition rate of AChE/N-CA@Au-[BSMIM]HSO_(4)/BDD,and the detection limit was 1.9674×10^(-11) g/L(calculated according to the inhibition rate of 10%),the linear range was wide,detection limit was low,stability and reproducibility was good,and the sensor had a good anti-interference ability to common heavy metal ions.AChE/N-CA@Au-[BSMIM]HSO_(4)/BDD was simple,convenient and fast,can be used for rapid quantitative analysis of dichlorvos.

关 键 词：敌敌畏 酶抑制 炭气凝胶 离子液体 金纳米材料 传感器 

分 类 号：TS255.7[轻工技术与工程—农产品加工及贮藏工程] TP212[轻工技术与工程—食品科学与工程]