牡丹根腐病致病菌株的分离及鉴定的研究  

The Isolation and Identification of Pathogenic Strains of Peony Root Rot

作　　者：郭燕风[1] 宋磊[1] 王传宝[1] 王蕊 孟国庆[1] GUO Yan-feng;SONG Lei;WANG Chuan-bao;WANG Rui;MENG Guo-qing(School of Agricultural and Biological Engineering,Heze University,Heze Shandong 274000,China;Shandong Center for Food and Drug Evaluation&Inspection,Jinan Shandong 250013,China)

机构地区：[1]菏泽学院农业与生物工程学院,山东菏泽274015 [2]山东省食品药品审评查验中心,山东济南250013

出　　处：《菏泽学院学报》2023年第2期116-118,共3页Journal of Heze University

基　　金：山东省重点研发计划(2021SFGC1205);山东省高校科技计划项目(J17KA125)。

摘　　要：以菏泽牡丹园多年生根腐病牡丹植株为研究对象,取其根部和根际土壤,进行梯度稀释,筛选获得1株致病菌GH-1,对其进行ITS序列克隆测序,并进行系统进化树构建,结合孢子形态,初步鉴定为镰刀菌属(Fusarium sp.),明确了菏泽牡丹根腐病的原因,为进一步防治牡丹根腐病提供基础.This paper selects tree peonies with root rot in Heze Peony Garden,takes roots and rhizosphere soil for gradient dilution,and obtains a pathogenic bacterium GH-1.Then,ITS sequence cloning and phylogenetic tree construction are performed on it.Combined with spore morphology,it was preliminarily identified as Fusarium sp.,which identifies the cause of root rot and provides a basis for further prevention and control of the disease.

关 键 词：根腐病 ITS序列 Fusarium sp. 

分 类 号：S436.8[农业科学—农业昆虫与害虫防治]