消渴舒方对2型糖尿病模型大鼠血清炎症因子的影响及其机制研究  

Effects of Xiaokeshu recipe on levels of serum inflammatory factors in type2 diabetic rats and its mechanism

作　　者：侯瑞英[1] 郭莉阁 焦伟杰[1] 杜蕾[1] 吴桂月 赵旭[1] HOU Ruiying;GUO Lige;JIAO Weijie;DU Lei;WU Guiyue;ZHAO Xu(Department of Pharmacy,Second Affiliated Hospital,Henan University of Chinese Medicine,Zhengzhou 450003,Henan,China;Department of Endocrine,Second Affiliated Hospital,Henan University of Chinese Medicine,Zhengzhou 450003,Henan,China)

机构地区：[1]河南中医药大学第二附属医院药学部,河南郑州450003 [2]河南中医药大学第二附属医院内分泌科,河南郑州450003

出　　处：《中国临床药理学与治疗学》2023年第4期377-382,共6页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：河南省中医药科学研究专项课题(20-21ZY2212);河南省中医药拔尖人才培养项目(豫卫中医函[2021]15号)。

摘　　要：目的:观察消渴舒方对2型糖尿病(type 2diabetes mellitus,T2DM)模型大鼠血清炎症因子及回肠组织Toll样受体4(toll-like receptor 4,TLR4)和核转录因子-κB(nuclear transcription factor-κB,NF-κB)的影响,探讨其治疗T2DM的可能机制。方法:SPF级SD雄性大鼠予高脂高糖饲料喂养联合腹腔注射链脲佐菌素(streptozotocin,STZ)制备2型糖尿病大鼠模型,并随机分为模型组、二甲双胍组、消渴舒方低、高剂量组,另设正常组。造模成功后,分别用二甲双胍和低、高剂量消渴舒方灌胃处理,正常组和模型组用生理盐水灌胃。观察治疗前后大鼠的一般状况;药物干预4周后,收集大鼠血清样本及回肠组织,分别进行生化和Western blot检测。结果:与模型组比较,消渴舒方低、高剂量和二甲双胍组大鼠多饮、多尿现象均得到不同程度改善。与模型组比较,消渴舒方低剂量组大鼠血清空腹血糖(fasting blood glucose,FBG)、空腹胰岛素(fasting insulin,FINS)和白细胞介素-1β(interleukin-1β,IL-1β)水平有所下降,但差异无统计学意义(P>0.05);消渴舒方高剂量和二甲双胍组FBG、FINS和IL-1β水平均明显下降,差异具有统计学意义(P<0.05或P<0.01);消渴舒方低、高剂量和二甲双胍组大鼠血清脂多糖(lipopolysaccharide,LPS)、白细胞介素-6(interleukin-6,IL-6)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)水平均显著降低,差异具有统计学意义(P<0.05或P<0.01)。与模型组比较,消渴舒方低、高剂量和二甲双胍组回肠组织TLR4、NF-κB蛋白表达均明显下调,差异具有统计学意义(P<0.05或P<0.01)。结论:消渴舒方可能通过降低血清LPS含量,抑制TLR4/NF-κB信号通路,减少炎症因子的释放,从而改善胰岛素抵抗、降低血糖。AIM:To explore the possible mechanism of Xiaokeshu recipe in the treatment of type 2 diabetes mellitus(T2DM)by observing the effects of Xiaokeshu Recipe on serum inflammatory factors.METHODS:Male SPF-grade SD rats were fed with high-fat and high-sugar fodder combined with intraperitoneal injection of streptozotocin(STZ)to prepare the model of T2DM.The model rats were randomly divided into a model group,a metformin group,low and high-dose Xiaokeshu recipe groups,and a normal group was set up.After successful modeling,the metformin group and the Xiaokeshu recipe groups were treated with metformin and Xiaokeshu recipe by gavage respectively,normal saline was given by gavage in the normal group and the model group.The general living status of rats before and after treatment was observed.After 4 weeks of drug intervention,serum samples and ileum tissue of rats were collected for biochemical and Western blot.RESULTS:As compaed with the model group,the polydipsia and polyuria in the low and high-dose Xiaokeshu recipe and the metformin groups could be improved.As compaed with the model group,the levels of fasting blood glucose(FBG),fasting insulin(FINS)and interleukin-1β(IL-1β)of rats in the low-dose Xiaokeshu recipe group were decreased,but the differences were statistically insignificant(P>0.05),the levels of FBG,FINS and IL-1βof rats in the high-dose Xiaokeshu recipe and the metformin groups were significantly decreased as compared with the the model group(P<0.05 or P<0.01).As compaed with the model group,the levels of Lipopolysaccharide(LPS),interleukin-6(IL-6),and tumor necrosis factor-α(TNF-α)in the high-dose Xiaokeshu recipe and the metformin groups were decreased(P<0.05 or P<0.01).As compared with the model group,the expressions of toll-like receptor 4(TLR4)and nuclear transcription factor(NF-κB)protein in ileal tissue were down-regulated in the low and high-dose Xiaokeshu Recipe and the metformin groups(P<0.05 or P<0.01).CONCLUSION:Xiaokeshu recipe may reduce the level of serum LPS,inhibit the TLR4/NF-κB

关 键 词：消渴舒方 2型糖尿病 炎症因子 TLR4/NF-κB信号通路 

分 类 号：R965.2[医药卫生—药理学]