神经细胞缺氧/复氧体外模型的研究进展  

Research progress on hypoxia/reoxygenation model of nerve cells in vitro

作　　者：王漫漫 雷震[2] 张勇[3] 付雪琴 邹旭欢 王玮玮 王保奇[4] 兰瑞[4] WANG Man-man;LEI Zhen;ZHANG Yong;FU Xue-qin;ZOU Xu-huan;WANG Wei-wei;WANG Bao-qi;LAN Rui(First Clinical Medical College,Henan University of Chinese Medicine,Zhengzhou 450056,China;Central Laboratory,the Second Affiliated Hospital,Henan University of Chinese Medicine,Zhengzhou 450056,China;Dept of Traditional Chinese Medicine,the Third Affiliated Hospital of Zhengzhou University,Zhengzhou 450014,China;Dept of Encephalopathy,the First Affiliated Hospital of Henan University of Chinese Medicine,Zhengzhou 450099,China)

机构地区：[1]河南中医药大学第一临床医学院,河南郑州450056 [2]河南中医药大学第二附属医院中心实验室,河南郑州450056 [3]郑州大学第三附属医院中医科,河南郑州450014 [4]河南中医药大学第一附属医院脑病科,河南郑州450099

出　　处：《中国药理学通报》2023年第5期823-828,共6页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81973618,81503422);河南省自然科学基金资助项目(No 202300410399)。

摘　　要：缺血性脑卒中是世界人口第二大死亡原因和第三大致残原因,发病率在全球范围内逐年增加。缺血性脑卒中经过血液再通治疗后会引起缺血/再灌注损伤,而缺血/再灌注损伤的机制目前仍不是十分明确,因此,构建具有其特性的临床前模型十分必要。鉴于动物模型研究的复杂性和体外脑切片培养的局限性,体外细胞模型成为了简化而有价值的工具。该文对目前常用的神经细胞以及缺血/再灌注体外模型的构建方法、构建原理及相关研究进展进行阐述,以期为合理选择缺氧/复氧细胞模型进行脑缺血/再灌注的基础研究与药物筛选提供参考和依据。Ischemic stroke is the second leading cause of human death and the third reason of disability.Meanwhile,the incidence is rising year after year worldwide.Ischemic stroke could cause ischemia-reperfusion injury after blood recanalization treat-ment,but the mechanism of ischemia-reperfusion injury is still not very clear,so it is necessary to build a preclinical model with specific characteristics.Up to now,animal experiments have been still complicated,and the culture of brain slices has some limitations.The cell model in vitro has become a simplified and valuable tool widely used by researchers.The paper systematically summarizes the common type of nerve cells,and further analyzes establishment methods and principle,relevant research progress on the in vitro model of ischemia-reperfusion,in order to provide reference for rationally selecting hypoxia and reoxygenation model for basic research on cerebral ischemia and reperfusion and drug screening.

关 键 词：神经细胞 缺氧/复氧 缺血/再灌注 细胞模型 构建方法 模型特点 

分 类 号：R322.8[医药卫生—人体解剖和组织胚胎学] R442.9[医药卫生—基础医学] R743.3R845.22R852.15