环介导等温扩增在动物病原微生物检测中的应用研究进展  

Application of loop-mediated isothermal amplification in the detection of animal pathogenic microorganisms

作　　者：唐雨萌 申秋平 宋春雷 庄林林 Tang Yumeng;Shen Qiuping;Song Chunlei;Zhuang Linlin(Jiangsu Vocational College of Agriculture and Forestry,Jiangsu Zhenjiang 212400)

机构地区：[1]江苏农林职业技术学院,江苏镇江212400

出　　处：《现代畜牧兽医》2023年第4期72-76,共5页Modern Journal of Animal Husbandry and Veterinary Medicine

基　　金：江苏农林职业技术学院青年扶持项目(2022kj32);江苏省高等学校基础科学(自然科学)研究面上项目(22KJB180001)。

摘　　要：病原微生物是威胁畜禽健康的重要因素,严重影响畜牧业的发展,快速、精准的病原微生物检测方法对于保障畜禽健康具有重要意义。环介导等温扩增(loop-mediated isothermal amplification,LAMP)是一种核酸等温扩增技术,具有灵敏度高、反应速度快、操作便捷等特点,原理是针对靶基因的6个区域设计2~3对引物,使用链置换DNA聚合酶即可实现高效扩增。文章对LAMP的反应机制、研究现状以及在动物病原微生物检测中的应用进展进行综述,并对其未来发展进行展望。Pathogenic microorganisms are an important factor threatening the health of livestock and poultry,which seriously affects the development of animal husbandry.Rapid and accurate detection methods of pathogenic microorganisms are of great significance to ensure the health of livestock and poultry.LAMP is one kind of nucleic acid isothermal amplification technique,which shows high sensitivity,rapidness and rapidness.The principle is to design two or three primers in six regions of the target genes.Efficient amplification can be achieved by using strand replacement DNA polymerase.In the paper,the mechanism,research status and application of LAMP in the detection of pathogenic microorganisms were reviewed,and the future development of LAMP was prospected.

关 键 词：环介导等温扩增技术 动物病原微生物 核酸检测 

分 类 号：S854.43[农业科学—临床兽医学]